铜绿假单胞菌在正常皮肤提取物和烧伤皮肤提取物中的生长：细胞外蛋白酶的作用
Growth of Pseudomonas aeruginosa in normal and burned skin extract: role of extracellular proteases.
作者信息
Cicmanec J F, Holder I A
出版信息
Infect Immun. 1979 Aug;25(2):477-83. doi: 10.1128/iai.25.2.477-483.1979.
Abstract
Growth curves and mean generation times (MGT) were determined for Pseudomonas aeruginosa strain M-2 (protease +) and strain PA-103 (protease +/-) in burned skin extract (BSE) and in normal skin extract (NSE). Strain M-2 grew on NSE or BSE with an MGT of 30 min. Strain PA-103 grew in NSE at a similar MGT; however, PA-103 in BSE had a MGT of 65 min. When protease was added to BSE, PA-103 grew as rapidly as M-2. When ammonium sulfate was added to inhibit protease production, the MGT of M-2 slowed to that of M2 in both BSE in NSE. The MGT of PA-103 in amino acid-supplemented BSE was similar to that of PA-103 in BSE. The MGT of PA-103 in amino acid-supplemented BSE was similar to that of M-2 in both BSE andNSE. These data suggest that protease may serve as a virulence factor by modifying the available nutrients in burned skin. As a result, nutrients are formed that permit an enhanced growth rate and amore rapid establishment of the infection in the host.
摘要
测定了铜绿假单胞菌M - 2菌株（蛋白酶阳性）和PA - 103菌株（蛋白酶弱阳性）在烧伤皮肤提取物（BSE）和正常皮肤提取物（NSE）中的生长曲线及平均世代时间（MGT）。M - 2菌株在NSE或BSE上生长，MGT为30分钟。PA - 103菌株在NSE中以相似的MGT生长；然而，PA - 103菌株在BSE中的MGT为65分钟。当向BSE中添加蛋白酶时，PA - 103菌株的生长速度与M - 2菌株一样快。当添加硫酸铵以抑制蛋白酶产生时，M - 2菌株在BSE和NSE中的MGT均减慢至与PA - 103菌株在BSE中的MGT相似。在补充氨基酸的BSE中，PA - 103菌株的MGT与在BSE中的相似。在补充氨基酸的BSE中，PA - 103菌株的MGT与在BSE和NSE中的M - 2菌株的相似。这些数据表明，蛋白酶可能通过改变烧伤皮肤中可用的营养物质而作为一种毒力因子。结果，形成了能使生长速率提高并在宿主体内更快速建立感染的营养物质。
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