Deaton C D, Maxwell K W, Smith R S, Creveling R L
Clin Chem. 1976 Sep;22(9):1465-71.
Measurement of immunoglobulins (Ig) and of complement component C3 in human serum by automated and manual nephelometric techniques is tedious, and the effective linear range is too narrow. We describe a laser nephelometer/reagent system for measuring serum proteins by the use of forward light scatter (which enhances the ratio of reaction/blank), electronic blank subtraction, laser light (632 nm), and electronic signal selection. We report data establishing the range of linearity for immunoglobulins IgG, IgA, IgM, and complement C3 with this system, and correlations with results by a radial immunodiffusion. We also compared an electroimmunodiffusion system for quantitation of albumin and IgG in cerebrospinal fluid to our technique. The nephelometric system described provides a rapid, accurate, precise, and objective way to measure immunoglobulins and C3.
采用自动和手动散射比浊技术测定人血清中的免疫球蛋白（Ig）和补体成分C3既繁琐，有效线性范围又太窄。我们描述了一种激光散射比浊仪/试剂系统，该系统通过利用前向光散射（可提高反应/空白比）、电子空白扣除、激光（632nm）和电子信号选择来测量血清蛋白。我们报告了用该系统确定免疫球蛋白IgG、IgA、IgM和补体C3线性范围的数据，以及与放射免疫扩散结果的相关性。我们还将用于定量脑脊液中白蛋白和IgG的电免疫扩散系统与我们的技术进行了比较。所描述的散射比浊系统为测量免疫球蛋白和C3提供了一种快速、准确、精确且客观的方法。
