Chang S T, Wu J H, Wang S Y, Kang P L, Yang N S, Shyur L F
Department of Forestry, National Taiwan University, Taipei 106, Taiwan.
J Agric Food Chem. 2001 Jul;49(7):3420-4. doi: 10.1021/jf0100907.
The antioxidant activity of extracts from bark and heartwood of Acacia confusa was evaluated by various antioxidant assays, including free radical and superoxide radical scavenging assays and lipid peroxidation assay as well as hydroxyl radical-induced DNA strand scission assay. In addition, an ex vivo antioxidant assay using a flow cytometric technique was also employed in this study. The results indicate that both bark and heartwood extracts clearly have strong antioxidant effects. Similar inhibitory activities for each test sample were found for both 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical generation and lipid peroxidation. As for the superoxide radical scavenging activity, the heartwood extract was more effective than the bark extract. Furthermore, the heartwood extract protected PhiX174 supercoiled DNA against strand scission induced by ultraviolet photolysis of H2O2, and it reduced the amounts of intracellular hydrogen peroxide, a reactive oxygen species, when it was co-incubated with human promyelocytic leukemia (HL-60) cells under oxidative stress.
通过各种抗氧化测定方法，包括自由基和超氧阴离子自由基清除测定、脂质过氧化测定以及羟基自由基诱导的DNA链断裂测定，对相思树木皮和心材提取物的抗氧化活性进行了评估。此外，本研究还采用了流式细胞术技术进行体外抗氧化测定。结果表明，树皮和心材提取物均具有明显的强抗氧化作用。对于1,1-二苯基-2-苦基肼（DPPH）自由基生成和脂质过氧化，每个测试样品都表现出类似的抑制活性。至于超氧阴离子自由基清除活性，心材提取物比树皮提取物更有效。此外，心材提取物可保护PhiX174超螺旋DNA免受H2O2紫外线光解诱导的链断裂，并且当它与处于氧化应激状态的人早幼粒细胞白血病（HL-60）细胞共同孵育时，可减少细胞内活性氧过氧化氢的量。
