Thapa Ishor, Pandey Ashmita, Tiwari Sunil, Awal Suvash Chandra
Department of Biotechnology, SANN International College, Purbanchal University, Biratnagar 56600, Nepal.
Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Curr Issues Mol Biol. 2025 Aug 5;47(8):624. doi: 10.3390/cimb47080624.
Wild from Nepal's high-altitude regions was studied to identify key bioactive compounds and assess the influence of solvent type-water, ethanol, methanol, and acetone-on extraction efficiency and biological activity. Extracts were evaluated for antioxidant potential, cytotoxicity against HeLa cells, and phytochemical composition via gas chromatography-mass spectrometry (GC-MS). Solvent type significantly affected both yield and bioactivity. Acetone yielded the highest crude extract (5.01%), while ethanol extract exhibited the highest total phenolic (376.5 ± 9.3 mg PG/g) and flavonoid content (30.3 ± 0.5 mg QE/g). Methanol extract was richest in lycopene (0.07 ± 0.00 mg/g) and β-carotene (0.45 ± 0.02 mg/g). Ethanol extract demonstrated consistently strong DPPH, superoxide, hydroxyl, and nitric oxide radical scavenging activity, along with high reducing power. All extracts showed dose-dependent cytotoxicity against HeLa cells, with ethanol and water extracts showing the greatest inhibition (>65% at 1000 µg/mL). GC-MS profiling identified solvent-specific bioactive compounds including sterols, terpenoids, polyphenols, and fatty acids. Notably, pharmacologically relevant compounds such as hinokione, ferruginol, ergosterol, and geranylgeraniol were detected. These findings demonstrate the therapeutic potential of , underscore the importance of solvent selection, and suggest that high-altitude ecological conditions may influence its bioactive metabolite profile.
对来自尼泊尔高海拔地区的[某种植物，原文未明确写出]进行了研究，以确定关键生物活性化合物，并评估溶剂类型（水、乙醇、甲醇和丙酮）对提取效率和生物活性的影响。通过气相色谱 - 质谱联用（GC - MS）对提取物的抗氧化潜力、对HeLa细胞的细胞毒性和植物化学成分进行了评估。溶剂类型对产量和生物活性均有显著影响。丙酮得到的粗提物产量最高（5.01%），而乙醇提取物的总酚含量最高（376.5±9.3毫克没食子酸丙酯/克）和黄酮含量最高（30.3±0.5毫克槲皮素当量/克）。甲醇提取物的番茄红素（0.07±0.00毫克/克）和β - 胡萝卜素（0.45±0.02毫克/克）含量最丰富。乙醇提取物表现出持续较强的DPPH、超氧阴离子、羟基和一氧化氮自由基清除活性，以及较高的还原能力。所有提取物对HeLa细胞均表现出剂量依赖性细胞毒性，乙醇和水提取物的抑制作用最大（在1000微克/毫升时>65%）。GC - MS分析确定了溶剂特异性生物活性化合物，包括甾醇、萜类、多酚和脂肪酸。值得注意的是，检测到了诸如扁柏酮、铁锈醇、麦角固醇和香叶基香叶醇等具有药理相关性的化合物。这些发现证明了[某种植物，原文未明确写出]的治疗潜力，强调了溶剂选择的重要性，并表明高海拔生态条件可能会影响其生物活性代谢产物谱。
