Abourashed E A, Khan I A
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA.
J Pharm Sci. 2001 Jul;90(7):817-22. doi: 10.1002/jps.1035.
Goldenseal (Hydrastis canadensis L., Ranunculaceae) is an ingredient of various dietary supplements intended for enhancing general body immunity. Many goldenseal products are currently available in the United States, either alone or in combination with echinacea. In most products, the content of the main active alkaloids of goldenseal, hydrastine and berberine, is not indicated on the label. A high-performance liquid chromatography (HPLC) method has been developed for the detection and quantification of hydrastine and berberine in a number of products obtained from the United States market. The method uses a Phenomenex Luna C(18) column, a mobile phase consisting of solvent A (100 mM sodium acetate/acetic acid, pH 4.0) and solvent B (acetonitrile/methanol; 90/10, v/v). Elution was run at a flow rate of 1.0 mL/min, with a linear gradient of 80- 40% A in B over 20 min and ultraviolet detection at 290 nm. A wide range of content variation was observed for both alkaloids in the tested samples.
白毛茛（北美黄连，毛茛科）是各种旨在增强全身免疫力的膳食补充剂的一种成分。目前在美国有许多白毛茛产品，单独或与紫锥菊属植物组合销售。在大多数产品中，标签上并未标明白毛茛主要活性生物碱——白毛茛碱和小檗碱的含量。已开发出一种高效液相色谱（HPLC）方法，用于检测和定量从美国市场获得的多种产品中的白毛茛碱和小檗碱。该方法使用菲罗门月神C(18) 柱，流动相由溶剂A（100 mM醋酸钠/醋酸，pH 4.0）和溶剂B（乙腈/甲醇；90/10，v/v）组成。以1.0 mL/min的流速进行洗脱，在20分钟内从80 - 40%的A（在B中）进行线性梯度洗脱，并在290 nm处进行紫外检测。在所测试的样品中，两种生物碱均观察到广泛的含量变化。
