利用随机扩增多态性DNA对枸杞与其近缘枸杞属物种进行鉴别。

Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA.

作者信息

Zhang K Y, Leung H W, Yeung H W, Wong R N

出版信息

Planta Med. 2001 Jun;67(4):379-81. doi: 10.1055/s-2001-14310.

DOI:10.1055/s-2001-14310

Abstract

The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.

摘要

首次应用随机扩增多态性DNA（RAPD）技术来区分枸杞与同属其他近缘物种。在本研究中，采集了8个样本，包括5个物种、2个变种和1个栽培变种。RAPD分析中共使用了50个随机引物。从10个引物中成功获得了对应不同枸杞物种的独特DNA指纹图谱。相似性指数（S.I.）分析表明，种内的值高于种间的值。这些结果证实，RAPD技术可用于区分枸杞的近缘物种。