Arif I A, Bakir M A, Khan H A, Al Farhan A H, Al Homaidan A A, Bahkali A H, Al Sadoon M, Shobrak M
Molecular Fingerprinting and Biodiversity Unit, Prince Sultan Research Chair for Environment and Wildlife, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
Genet Mol Res. 2010 Nov 9;9(4):2191-8. doi: 10.4238/vol9-4gmr848.
The use of highly discriminatory methods for the identification and characterization of genotypes is essential for plant protection and appropriate use. We utilized the RAPD method for the genetic fingerprinting of 11 plant species of desert origin (seven with known medicinal value). Andrachne telephioides, Zilla spinosa, Caylusea hexagyna, Achillea fragrantissima, Lycium shawii, Moricandia sinaica, Rumex vesicarius, Bassia eriophora, Zygophyllum propinquum subsp migahidii, Withania somnifera, and Sonchus oleraceus were collected from various areas of Saudi Arabia. The five primers used were able to amplify the DNA from all the plant species. The amplified products of the RAPD profiles ranged from 307 to 1772 bp. A total of 164 bands were observed for 11 plant species, using five primers. The number of well-defined and major bands for a single plant species for a single primer ranged from 1 to 10. The highest pair-wise similarities (0.32) were observed between A. fragrantissima and L. shawii, when five primers were combined. The lowest similarities (0) were observed between A. telephioides and Z. spinosa; Z. spinosa and B. eriophora; B. eriophora and Z. propinquum. In conclusion, the RAPD method successfully discriminates among all the plant species, therefore providing an easy and rapid tool for identification, conservation and sustainable use of these plants.
使用高度 discriminatory 方法来鉴定和表征基因型对于植物保护和合理利用至关重要。我们利用随机扩增多态性DNA（RAPD）方法对11种沙漠起源的植物物种（其中7种具有已知药用价值）进行遗传指纹分析。Andrachne telephioides、Zilla spinosa、Caylusea hexagyna、Achillea fragrantissima、Lycium shawii、Moricandia sinaica、Rumex vesicarius、Bassia eriophora、Zygophyllum propinquum subsp migahidii、Withania somnifera和Sonchus oleraceus从沙特阿拉伯的不同地区采集。所使用的5种引物能够扩增所有植物物种的DNA。RAPD图谱的扩增产物范围为307至1772碱基对。使用5种引物对11种植物物种共观察到164条带。单个引物对单个植物物种明确的主带数量范围为1至10条。当5种引物组合使用时，在A. fragrantissima和L. shawii之间观察到最高的成对相似性（0.32）。在A. telephioides和Z. spinosa之间；Z. spinosa和B. eriophora之间；B. eriophora和Z. propinquum之间观察到最低的相似性（0）。总之，RAPD方法成功地区分了所有植物物种，因此为这些植物的鉴定、保护和可持续利用提供了一种简便快速的工具。 （注：“discriminatory”这里可能是“区分性的”意思，结合语境翻译，但不确定该词是否准确，原文可能有误，正常应该是“discriminating”之类的词）
