[苯妥英钠对人精子染色体结构畸变的体外作用]
[Effects of phenytoin on structural aberration of human sperm chromosomes in vitro].
作者信息
Meng H, Zhang F, Gao X, Wang X, Li D, Cui X, Wang Z
机构信息
Department of Psychiatry, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016 P.R.China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001 Aug;18(4):303-5.
OBJECTIVE
To detect the mutagenic effects of phenytoin on human sperm chromosomes.
METHODS
The mutagenic effects of phenytoin at 10, 20 and 40 microgram/ml were tested by an in vitro testing system of human sperm chromosomes, with leomycin A5 as a positive control and mutagen-free solution as the blank control.
RESULTS
The frequencies of sperm with structural chromosomal aberrations and breakages of sperm chromosomes in the groups of 10 microgram/ml, 20 microgram/ml and 40 ug/ml of phenytoin were all higher than those in the blank control, but only in the group of 40 ug/ml, the elevated frequencies were statistically significant (P<0.005, P<0.05).
CONCLUSION
Phenytoin has probably mutagenic potential effect on human sperm cells.
目的
检测苯妥英对人类精子染色体的致突变作用。
方法
采用人类精子染色体体外检测系统，检测10、20和40微克/毫升苯妥英的致突变作用，以丝裂霉素A5作为阳性对照，无诱变剂溶液作为空白对照。
结果
10微克/毫升、20微克/毫升和40微克/毫升苯妥英组精子染色体结构畸变和精子染色体断裂的频率均高于空白对照组，但仅40微克/毫升组频率升高具有统计学意义（P<0.005，P<0.05）。
结论
苯妥英可能对人类精子细胞有致突变潜在作用。
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