Pauletto Marianna, Elgendy Ramy, Ianni Andrea, Marone Elettra, Giantin Mery, Grotta Lisa, Ramazzotti Solange, Bennato Francesca, Dacasto Mauro, Martino Giuseppe
Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020 Legnaro (PD), Italy.
Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Animals (Basel). 2020 Apr 19;10(4):714. doi: 10.3390/ani10040714.
The increasing demand for more animal products put pressure on improving livestock production efficiency and sustainability. In this context, advanced animal nutrition studies appear indispensable. Here, the effect of grape pomace (GP), the polyphenol-rich agricultural by-product, was evaluated on Holstein-Friesian cows' whole-blood transcriptome, milk production and composition. Two experimental groups were set up. The first one received a basal diet and served as a control, while the second one received a 7.5% GP-supplemented diet for a total of 60 days. Milk production and composition were not different between the group; however, the transcriptome analysis revealed a total of 40 genes significantly affected by GP supplementation. Among the most interesting down-regulated genes, we found the DnaJ heat-shock protein family member A1 (DNAJA1), the mitochondrial fission factor (MFF), and the impact RWD domain protein (IMPACT) genes. The gene set enrichment analysis evidenced the positive enrichment of 'interferon alpha (IFN-α) and IFN-γ response', 'IL6-JAK-STAT3 signaling' and 'complement' genes. Moreover, the functional analysis denoted positive enrichment of the 'response to protozoan' and 'negative regulation of viral genome replication' biological processes. Our data provide an overall view of the blood transcriptomic signature after a 60-day GP supplementation in dairy cows which mainly reflects a GP-induced immunomodulatory effect.
对更多动物产品的需求不断增加，给提高畜牧生产效率和可持续性带来了压力。在这种背景下，先进的动物营养研究显得不可或缺。在此，评估了富含多酚的农业副产品葡萄皮渣（GP）对荷斯坦-弗里生奶牛全血转录组、产奶量和奶成分的影响。设置了两个实验组。第一个组接受基础日粮并作为对照，而第二个组接受添加7.5% GP的日粮，共60天。两组之间的产奶量和奶成分没有差异；然而，转录组分析显示共有40个基因受到GP添加的显著影响。在最有趣的下调基因中，我们发现了DnaJ热休克蛋白家族成员A1（DNAJA1）、线粒体分裂因子（MFF）和冲击RWD结构域蛋白（IMPACT）基因。基因集富集分析证明了“干扰素α（IFN-α）和IFN-γ反应”、“IL6-JAK-STAT3信号传导”和“补体”基因的正富集。此外，功能分析表明“对原生动物的反应”和“病毒基因组复制的负调控”生物过程的正富集。我们的数据提供了奶牛在补充60天GP后血液转录组特征的总体情况，这主要反映了GP诱导的免疫调节作用。
