Rodan G A, Mensi T, Harvey A
Calcif Tissue Res. 1975 Jul 25;18(2):125-31. doi: 10.1007/BF02546233.
A quantitative method for the application of compressive forces to long bones in culture is described. Using this method it was found that a physiological pressure of 80g/cm sq. applied to tibiae of 16-day-old chick embryos reduced glucose consumption to 50% of controls. Twenty four hours after the release of pressure glucose utilization again increased, approaching control levels. A pressure of 80 g/cm sq. also stimulated thymidine incorporation into DNA. The same pressure decreased the size of the extracellular fluid pool by 8%, but had no effect on the rate of equilibration of this pool with 22Na.
本文描述了一种在培养过程中对长骨施加压缩力的定量方法。使用该方法发现，对16日龄鸡胚胫骨施加80g/cm²的生理压力可使葡萄糖消耗量降至对照组的50%。压力释放24小时后，葡萄糖利用率再次增加，接近对照水平。80g/cm²的压力还刺激了胸苷掺入DNA。相同压力使细胞外液池大小减少了8%，但对该池与²²Na的平衡速率没有影响。
