Saeed I, Roepstorff A, Rasmussen T, Høg M, Jungersen G
Danish Centre For Experimental Parasitology, Agricultural University, Frederiksberg, Denmark.
Acta Vet Scand. 2001;42(2):279-86. doi: 10.1186/1751-0147-42-279.
Experiments on use of an agar-gel method for recovery of migrating Ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. Subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1% agar and the larvae allowed to migrate out of the agar-gel into 0.9% NaCl at 38 degrees C. The results showed that within 3 h more than 88% of the recoverable larvae migrated out of the liver agar-gel and more than 83% of the obtained larvae migrated out of the lung agar-gel. The larvae were subsequently available in a very clean suspension which reduced the sample counting time. Blending the liver for 60 sec in a commercial blender showed significantly higher larvae recovery than blending for 30 sec. Addition of gentamycin to reduce bacterial growth during incubation, glucose to increase larval motility during migration or ice to increase sedimentation of migrated larvae did not influence larvae recovery significantly.
为了获得快速标准化的方法，开展了关于使用琼脂凝胶法从猪的肝脏和肺中回收迁移的猪蛔虫幼虫的实验。将猪肝和肺的混合组织子样本与琼脂混合，使琼脂最终浓度达到1%，并让幼虫在38摄氏度下从琼脂凝胶中迁移到0.9%的氯化钠溶液中。结果表明，在3小时内，超过88%可回收的幼虫从肝脏琼脂凝胶中迁移出来，超过83%获得的幼虫从肺琼脂凝胶中迁移出来。随后幼虫存在于非常纯净的悬浮液中，这减少了样本计数时间。在商用搅拌机中将肝脏搅拌60秒显示出比搅拌30秒显著更高的幼虫回收率。添加庆大霉素以减少培养过程中的细菌生长、添加葡萄糖以增加迁移过程中幼虫的活力或添加冰以增加迁移幼虫的沉降对幼虫回收率没有显著影响。
