Slotved H C, Barnes E H, Eriksen L, Roepstorff A, Nansen P, Bjørn H
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Acta Vet Scand. 1997;38(3):207-12. doi: 10.1186/BF03548483.
Four groups each of 3 pigs were inoculated with Ascaris suum eggs. Pigs in groups 1 and 3 were inoculated with 1000 eggs, and pigs in groups 2 and 4 with 10,000 eggs. On day 10 and 21 post-inoculation (p.i.), respectively, groups 1 + 2 and 3 + 4 were slaughtered, and the contents from the small intestines collected. The contents were mixed with agar to a final concentration of 1% agar and allowed to sediment. The larvae were allowed to migrate from the agar-gel into 38 degrees C 0.9% saline overnight, and were then collected on a sieve (20 microns mesh) and counted. The larvae retained in the agar-gel were counted after pouring the melted agar through a sieve (20 microns mesh). The results showed that more than 97% of the larvae migrated out of the agar-gel and were available for counting in an almost clean suspension. The inoculation dose level did not significantly affect the recovery percentage, neither did the larval stage (10 or 21 days old larvae). The variation in the time interval from slaughtering to start of incubation (interval 57-155 min) did not significantly affect the recovery percentage.
将四组每组3头猪接种猪蛔虫卵。第1组和第3组的猪接种1000枚卵，第2组和第4组的猪接种10000枚卵。分别在接种后第10天和第21天，宰杀第1 + 2组和第3 + 4组的猪，并收集小肠内容物。将内容物与琼脂混合至最终浓度为1%琼脂，然后使其沉淀。让幼虫从琼脂凝胶迁移到38摄氏度的0.9%盐水中过夜，然后用筛网（20微米筛孔）收集并计数。将融化的琼脂通过筛网（20微米筛孔）后，对保留在琼脂凝胶中的幼虫进行计数。结果表明，超过97%的幼虫从琼脂凝胶中迁移出来，可在几乎纯净的悬浮液中进行计数。接种剂量水平对回收率没有显著影响，幼虫阶段（10日龄或21日龄幼虫）也没有影响。从宰杀到开始孵化的时间间隔变化（间隔57 - 155分钟）对回收率没有显著影响。
