van Wyk J A, Gerber H M, Groeneveld H T
Onderstepoort J Vet Res. 1980 Sep;47(3):147-58.
A gelled-agar technique for worm recovery was adapted to facilitate the recovery of larval and adult nematodes from the total ingesta of large numbers of sheep. The technique was also used to recover nematodes from 4 calves. In one trial involving 120 sheep, 100% of 2013 4th stage larvae (L4) and 92,1% of 134,205 adult Haemonchus contortus migrated from the agar preparations. Highly significantly more male than female worms failed to migrate. Using 1 x 1/10 aliquot to estimate the numbers of worms that failed to migrate from the agar, the mean error in the total worm count (worms that migrated plus those that failed to migrate) per sheep was 2,2%; with an examination of 2 x 1/10 aliquot the error was 1,7%. We concluded from this that the gelled-agar method may be of value for quantitative worm recovery, for example, in anthelmintic tests. In a second trial, 98,5% of 17,056 L4 and adult nematodes of 5 genera migrated from the ingesta of 4 calves and 96,4% of 62,597 L4 and adult nematodes of 9 species from the ingesta of 15 sheep. In general, L4 migrated slightly more efficiently than adult worms. In sheep and, to a lesser extent, in calves, Haemonchus spp. did not migrate as efficiently as the other genera such as Ostertagia, Trichostrongylus, Nematodirus, Oesophagostomum, Marshallagia and Chabertia.
一种用于蠕虫回收的凝胶琼脂技术被加以改进，以利于从大量绵羊的全部食入物中回收幼虫和成虫线虫。该技术还用于从4头犊牛中回收线虫。在一项涉及120只绵羊的试验中，2013条第四期幼虫（L4）中的100%以及134205条成年捻转血矛线虫中的92.1%从琼脂制剂中迁移出来。未能迁移的雄虫数量显著多于雌虫。使用1×1/10的等分试样来估计未能从琼脂中迁移出来的蠕虫数量，每只绵羊总蠕虫计数（迁移的蠕虫加上未能迁移的蠕虫）的平均误差为2.2%；检查2×1/10的等分试样时，误差为1.7%。我们由此得出结论，凝胶琼脂法可能在定量蠕虫回收方面具有价值，例如在驱虫试验中。在第二项试验中，17056条5个属的L4和成虫线虫中的98.5%从4头犊牛的食入物中迁移出来，62597条9个物种的L4和成虫线虫中的96.4%从15只绵羊的食入物中迁移出来。一般来说，L4的迁移效率略高于成虫。在绵羊中，以及在较小程度上在犊牛中，血矛线虫属的迁移效率不如奥斯特他线虫属、毛圆线虫属、细颈线虫属、食道口线虫属、马歇尔线虫属和夏伯特线虫属等其他属。
