Slotved H C, Roepstorff A, Barnes E H, Eriksen L, Nansen P
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
J Parasitol. 1996 Aug;82(4):612-5.
Nine groups of 5 pigs were inoculated with Ascaris suum eggs on day 0. Groups 1, 2, and 3 were inoculated with 100 eggs, groups 4, 5, and 6 with 1,000 eggs, and groups 7, 8, and 9 with 10,000 eggs. On day 3, groups 1, 4, and 7 were slaughtered, on day 7 groups 2, 5, and 8, and on day 10 groups 3, 6, and 9. The liver (days 3 and 7) and lungs (days 3, 7, and 10) were removed and 2, 25% samples of both organs were collected. Larvae were recovered from 1 sample by the Baermann method and from the other by an agar-gel method. Overall there were no significant differences in the liver larval recovery between the 2 methods. The use of the agar-gel method resulted in a very clean suspension of larvae and thereby reduced the sample counting time by a factor of 5-10 compared to the Baermann method. With both methods larval recovery from the lungs resulted in a clean larval suspension that was easy to count, and there were overall no significant differences between the 2 methods, although there was a tendency toward the Baermann method recovering more larvae from the lungs than the agar-gel method. The tissue sample dry weight did not significantly influence larval recovery by the agar-gel method, and the time interval from slaughtering to start of incubation on day 3 (interval 51-92 min), day 7 (interval 37-114 min), and day 10 (interval 50-129 min) had no significant effect on recovery by either method.
9组，每组5头猪在第0天接种猪蛔虫卵。第1、2和3组接种100枚卵，第4、5和6组接种1000枚卵，第7、8和9组接种10000枚卵。在第3天，宰杀第1、4和7组；在第7天，宰杀第2、5和8组；在第10天，宰杀第3、6和9组。取出肝脏（第3天和第7天）和肺脏（第3天、第7天和第10天），并采集这两个器官2个25%的样本。通过贝尔曼法从1个样本中回收幼虫，从另一个样本中通过琼脂凝胶法回收幼虫。总体而言，两种方法在肝脏幼虫回收率上没有显著差异。与贝尔曼法相比，琼脂凝胶法产生的幼虫悬浮液非常纯净，从而将样本计数时间减少了5至10倍。使用两种方法从肺脏中回收幼虫都得到了易于计数的纯净幼虫悬浮液，尽管有趋势表明贝尔曼法从肺脏中回收的幼虫比琼脂凝胶法多，但总体上两种方法之间没有显著差异。组织样本干重对琼脂凝胶法的幼虫回收率没有显著影响，并且在第3天（间隔51 - 92分钟）、第7天（间隔37 - 114分钟）和第10天（间隔50 - 129分钟）从宰杀到开始孵育的时间间隔对两种方法的回收率都没有显著影响。
