Martin S E, Iandolo J J
J Bacteriol. 1975 Jun;122(3):1136-43. doi: 10.1128/jb.122.3.1136-1143.1975.
The calculated in vivo polypeptide chain growth rate for Staphylococcus auteus MF-31 grown in nutritionally rich medium assuming all the ribosomes were functional was found to be approximately 16 amino acids/s/ribosome, but decreased to 10.2 amino acids/s/ribosome for cells grown in poor medium. An in vitro analysis revealed that 70S ribosomes isolated from rich medium cells were more active than similar 70S ribosomes derived from cells grown in poor medium. The 30S subunit was found responsible for the increased activity of the rich monosomes, whereas the 50S subunit appeared to be capable of either high or low activity.
假设所有核糖体都有功能,在营养丰富的培养基中生长的金黄色葡萄球菌MF-31的体内多肽链生长速率经计算约为16个氨基酸/秒/核糖体,但在营养贫乏的培养基中生长的细胞,该速率降至10.2个氨基酸/秒/核糖体。体外分析表明,从营养丰富的培养基中生长的细胞分离出的70S核糖体比从营养贫乏的培养基中生长的细胞分离出的类似70S核糖体更具活性。发现30S亚基是富含核糖体的单核糖体活性增加的原因,而50S亚基似乎具有高活性或低活性。