van Dijk-Salkinoja M S, Planta R J
J Bacteriol. 1971 Jan;105(1):20-7. doi: 10.1128/jb.105.1.20-27.1971.
The ribosome content of exponential-phase cells of Bacillus licheniformis was measured at three different growth rates. The average number of ribosomes per cell was about 92,000, 34,400, or 12,500 70S equivalents in balanced cultures growing at 37 C with generation times of 35, 60, and 120 min, respectively. Since the ribosomal particles were shown to be metabolically stable in exponentially growing cells, these figures implicate large differences in the quantity of ribosomes synthesized per unit of time in an individual cell grown under the various conditions. Nevertheless, the time required for the biosynthesis of a single 50S subunit was constant (about 10 min) and independent of the specific growth rate of the cell (within the limits studied). These results show that ribosome production is not regulated by control of the rate of assemblage of individual ribosomes, but rather by control of the number of the ribosomes in manufacture at a time.
在三种不同的生长速率下，测定了地衣芽孢杆菌指数生长期细胞的核糖体含量。在37℃下生长的平衡培养物中，代时分别为35分钟、60分钟和120分钟时，每个细胞中核糖体的平均数量分别约为92,000、34,400或12,500个70S当量。由于核糖体颗粒在指数生长的细胞中显示出代谢稳定，这些数字表明在不同条件下生长的单个细胞中，单位时间内合成的核糖体数量存在很大差异。然而，单个50S亚基生物合成所需的时间是恒定的（约10分钟），并且与细胞的比生长速率无关（在所研究的范围内）。这些结果表明，核糖体的产生不是通过控制单个核糖体的组装速率来调节的，而是通过控制一次制造的核糖体数量来调节的。
