Bengtsson M, Bäckman A C, Liblikas I, Ramirez M I, Borg-Karlson A K, Ansebo L, Anderson P, Löfqvist J, Witzgall P
Department of Crop Science, Swedish University of Agricultural Sciences, 230 53 Alnarp, Sweden.
J Agric Food Chem. 2001 Aug;49(8):3736-41. doi: 10.1021/jf0100548.
Volatile compounds were collected from apple branches (Malus domestica) at different developmental stages, and the antennal response of codling moth females (Cydia pomonella) to these compounds was recorded by electroantennography coupled to gas chromatography. Presence of a range of terpenoid compounds, many of which had antennal activity, was characteristic for volatile collections from branches with leaves, and from small green apples. Nine compounds from branches with leaves and green fruit consistently elicited an antennal response: methyl salicylate, (E)-beta-farnesene, beta-caryophyllene, 4,8-dimethyl-1,3(E),7-nonatriene, (Z)3-hexenol, (Z,E)-alpha-farnesene, linalool, germacrene D, and (E,E)-alpha-farnesene. The bouquet emitted from flowering branches contained in addition several benzenoid compounds which were not found after bloom. Small green apples, which are the main target of codling moth oviposition during the first seasonal flight period, released very few esters. In comparison, fully grown apples released a large number of esters, but fewer terpenoids. The study of apple volatiles eliciting an antennal response, together with a survey of the seasonal change in the release of these compounds, is the first step toward the identification of volatiles mediating host-finding and oviposition in codling moth females.
在不同发育阶段从苹果树枝（苹果属）收集挥发性化合物，并通过气相色谱联用触角电位记录法记录苹果蠹蛾雌虫（苹果小卷蛾）对这些化合物的触角反应。一系列萜类化合物的存在是带有叶片的树枝和小青苹果挥发性物质收集的特征，其中许多具有触角活性。来自带叶树枝和绿色果实的9种化合物始终引发触角反应：水杨酸甲酯、（E）-β-法尼烯、β-石竹烯、4,8-二甲基-1,3（E）,7-壬三烯、（Z）-3-己烯醇、（Z,E）-α-法尼烯、芳樟醇、吉马烯D和（E,E）-α-法尼烯。开花树枝散发的香气还含有几种在开花后未发现的苯类化合物。小青苹果是苹果蠹蛾在第一个季节性飞行期产卵的主要目标，释放的酯类很少。相比之下，成熟的苹果释放大量酯类，但萜类较少。研究引发触角反应的苹果挥发性物质，以及对这些化合物释放的季节性变化进行调查，是鉴定介导苹果蠹蛾雌虫寻找宿主和产卵的挥发性物质的第一步。
