Avsar F M, Sahin M, Aksoy F, Avsar A F, Aköz M, Hengirmen S, Bilici S
Ankara Numune Training Research and Hospital, Clinic of 1st Surgery, Ankara, Turkey.
Am J Surg. 2001 Jun;181(6):512-5. doi: 10.1016/s0002-9610(01)00617-1.
The purpose of this study was to determine the effects of diphenhydramine hydrochloride and methylprednisolone in peritoneal adhesions.
Forty-eight male rats were used in the study. The rats were anesthetized by 5 mg/kg ketamine hydrochloride. After opening the abdomen, 10 longitudinal incisions of 2 to 3 cm in length were made on the right parietal peritoneum, and a 2 cm(2) peritoneal layer was excised from the left abdominal wall. The abdomen was closed with 3/0 silk suture. Group I was the control group, group II was given 10 mg/kg diphenhydramine intravenously, group III was given 20 mg/kg methylprednisolone intravenously, and group IV was administered both of the drugs in the above doses. A blood sample of 2 mL was taken from the rats on the 14th day after the operation. The animals were then sacrificed. The abdomen was opened and abdominal adhesions were examined. A tissue sample of 1 g was taken from the abdominal incision line. Albumin, zinc, and hemoglobin levels and leucocyte counts in the blood were determined as well as hydroxyproline levels in the tissue.
Numbers of adhesions were as follows: 9 in group I, 3 in group II, and 2 in group III. No adhesion was observed in group IV. Albumin, zinc, and hemoglobin levels and leucocyte counts were found to be similar in all groups. Hydroxyproline levels in the tissue were significantly lower in groups III and IV than in groups I and II (P <0.05).
Diphenhydramine and methylprednisolone reduced postoperative adhesions significantly in rats. Further investigations are needed in order to use these drugs as antiadhesive agents in humans.
本研究旨在确定盐酸苯海拉明和甲泼尼龙对腹膜粘连的影响。
本研究使用了48只雄性大鼠。大鼠通过5mg/kg盐酸氯胺酮麻醉。打开腹腔后，在右顶叶腹膜上做10个长度为2至3厘米的纵向切口，并从左腹壁切除2平方厘米的腹膜层。用3/0丝线缝合关闭腹腔。第一组为对照组，第二组静脉注射10mg/kg苯海拉明，第三组静脉注射20mg/kg甲泼尼龙，第四组给予上述剂量的两种药物。术后第14天从大鼠采集2mL血液样本。然后处死动物。打开腹腔检查腹部粘连情况。从腹部切口线取1g组织样本。测定血液中的白蛋白、锌、血红蛋白水平和白细胞计数以及组织中的羟脯氨酸水平。
粘连数量如下：第一组9处，第二组3处，第三组2处。第四组未观察到粘连。发现所有组的白蛋白、锌、血红蛋白水平和白细胞计数相似。第三组和第四组组织中的羟脯氨酸水平显著低于第一组和第二组（P<0.05）。
苯海拉明和甲泼尼龙可显著减少大鼠术后粘连。为了将这些药物用作人类的抗粘连剂，还需要进一步研究。
