Saeed Mohammad F, Li Li, Wang Heiman, Weaver Scott C, Barrett Alan D T
Department of Microbiology & Immunology, 2Department of Pathology and 3Center for Tropical Diseases, The University of Texas Medical Branch, Galveston, TX 77555, USA.
J Gen Virol. 2001 Sep;82(Pt 9):2173-2181. doi: 10.1099/0022-1317-82-9-2173.
The Simbu serogroup of the genus Bunyavirus, family Bunyaviridae contains 25 viruses. Previous serological studies provided important information regarding some but not all of the relationships among Simbu serogroup viruses. This report describes the nucleotide sequence determination of the nucleocapsid (N) gene of the small genomic segment of 14 Simbu serogroup viruses and partial nucleotide sequence determination of the G2 glycoprotein-coding region (encoded by the medium RNA segment) of 19 viruses. The overall phylogeny of the Simbu serogroup inferred from analyses of the N gene was similar to that inferred from analyses of the G2 protein-coding region. Both analyses revealed that the Simbu serogroup viruses have evolved into at least five major phylogenetic lineages. In general, these phylogenetic lineages were consistent with the previous serological data, but provided a more detailed understanding of the relatedness amongst many viruses. In comparison to previous phylogenetic studies on the California and Bunyamwera serogroups of the Bunyavirus genus, the Simbu serogroup displays much larger genetic variation in the N gene (up to 40% amino acid sequence divergence).
布尼亚病毒科布尼亚病毒属的辛布血清群包含25种病毒。先前的血清学研究提供了关于辛布血清群病毒之间部分而非全部关系的重要信息。本报告描述了14种辛布血清群病毒小基因组片段核衣壳（N）基因的核苷酸序列测定，以及19种病毒G2糖蛋白编码区（由中RNA片段编码）的部分核苷酸序列测定。从N基因分析推断出的辛布血清群总体系统发育与从G2蛋白编码区分析推断出的相似。两种分析均表明，辛布血清群病毒已进化为至少五个主要的系统发育谱系。总体而言，这些系统发育谱系与先前的血清学数据一致，但对许多病毒之间的亲缘关系提供了更详细的了解。与先前对布尼亚病毒属加利福尼亚血清群和布尼亚姆韦拉血清群的系统发育研究相比，辛布血清群在N基因中显示出更大的遗传变异（氨基酸序列差异高达40%）。
