Shchetinin Alexey M, Lvov Dmitry K, Deriabin Petr G, Botikov Andrey G, Gitelman Asya K, Kuhn Jens H, Alkhovsky Sergey V
D.I. Ivanovsky Institute of Virology, Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Moscow, Russia.
Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, MD 21702, USA.
Viruses. 2015 Nov 23;7(11):5987-6008. doi: 10.3390/v7112918.
The family Bunyaviridae has more than 530 members that are distributed among five genera or remain to be classified. The genus Orthobunyavirus is the most diverse bunyaviral genus with more than 220 viruses that have been assigned to more than 18 serogroups based on serological cross-reactions and limited molecular-biological characterization. Sequence information for all three orthobunyaviral genome segments is only available for viruses belonging to the Bunyamwera, Bwamba/Pongola, California encephalitis, Gamboa, Group C, Mapputta, Nyando, and Simbu serogroups. Here we present coding-complete sequences for all three genome segments of 15 orthobunyaviruses belonging to the Anopheles A, Capim, Guamá, Kongool, Tete, and Turlock serogroups, and of two unclassified bunyaviruses previously not known to be orthobunyaviruses (Tataguine and Witwatersrand viruses). Using those sequence data, we established the most comprehensive phylogeny of the Orthobunyavirus genus to date, now covering 15 serogroups. Our results emphasize the high genetic diversity of orthobunyaviruses and reveal that the presence of the small nonstructural protein (NSs)-encoding open reading frame is not as common in orthobunyavirus genomes as previously thought.
布尼亚病毒科有530多个成员，分布在五个属中或有待分类。正布尼亚病毒属是最多样化的布尼亚病毒属，有220多种病毒，根据血清学交叉反应和有限的分子生物学特征，已被归入18多个血清群。仅对于属于布尼亚姆韦拉、布万巴/蓬戈拉、加利福尼亚脑炎、甘博阿、C组、马普塔、尼扬多和辛布血清群的病毒，才有所有三个正布尼亚病毒基因组片段的序列信息。在这里，我们展示了属于按蚊A、卡皮姆、瓜马、孔戈尔、太特和特洛克血清群的15种正布尼亚病毒以及两种以前未知为正布尼亚病毒的未分类布尼亚病毒（塔塔吉内病毒和威特沃特斯兰德病毒）的所有三个基因组片段的编码完整序列。利用这些序列数据，我们建立了迄今为止正布尼亚病毒属最全面的系统发育树，现在涵盖15个血清群。我们的结果强调了正布尼亚病毒的高度遗传多样性，并揭示了编码小非结构蛋白（NSs）的开放阅读框在正布尼亚病毒基因组中的存在并不像以前认为的那样常见。
