发育中大鼠和鸡肌管的膜及乙酰胆碱受体的电生理特性
Electrophysiological properties of the membrane and acetylcholine receptor in developing rat and chick myotubes.
作者信息
Ritchie A K, Fambrough D M
出版信息
J Gen Physiol. 1975 Sep;66(3):327-55. doi: 10.1085/jgp.66.3.327.
Abstract
Membrane properties of rat and chick myotubes in various stages of development were studied. Resting membrane potentials (Em) increased from -8 to -55 mV in both rat and chick as the myotubes developed from myoblasts to large multinucleated fibers. In the rat myotubes, this increase was not accompanied by significant changes in specific membrane resistivity or changes in Na+ and K+ ion distribution. Nor have we observed a significant electrogenic component to the resting Em of mature rat myotubues under normal circumstances. A progressive increase in the passive permeability of the membrane to K+ relative to Na+ ions has been observed which can account for the changes in Em with development. In contrast to the changes in the ionic selectivity of the membrane, we have found that the ionic selectivity of the ACh receptor of rat and chick myotubes remains constant during the same period of myotube development.
摘要
研究了大鼠和鸡肌管在不同发育阶段的膜特性。随着肌管从成肌细胞发育为大型多核纤维，大鼠和鸡的静息膜电位（Em）均从 -8 mV 增加到 -55 mV。在大鼠肌管中，这种增加并未伴随着比膜电阻率的显著变化或 Na⁺ 和 K⁺ 离子分布的变化。在正常情况下，我们也未观察到成熟大鼠肌管静息 Em 有显著的生电成分。已观察到膜对 K⁺ 相对于 Na⁺ 离子的被动通透性逐渐增加，这可以解释发育过程中 Em 的变化。与膜的离子选择性变化相反，我们发现大鼠和鸡肌管的乙酰胆碱受体的离子选择性在肌管发育的同一时期保持恒定。
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