钙与米氏巨轴突的相互作用以及基于简单表面电荷模型的描述。
The interactions of calcium with mpyxicola giant axons and a description in terms of a simple surface charge model.
作者信息
Schauf C L
出版信息
J Physiol. 1975 Jul;248(3):613-24. doi: 10.1113/jphysiol.1975.sp010991.
Abstract
- Myxicola giant axons were examined under voltage clamp in solutions with [Ca2+] ranging from 2 to 200 mM. 2. The magnitude of the shifts in the Na and K conductance-voltage curves, as well as membrane rate constants, caused by changes IN [Ca22+] were comparable and consistent with a model in which there exists a negative surface charge density of -0.013 charges/A-2, but there is no significant binding of divalent cations to the external membrane surface. 3. There was no effect of equimolar substitution of magnesium for Ca on the positions of the conductance-voltage curves along the voltage axis, consistent with the above conclusion. 4. Ca2+ has no effect on the shape of the steady-state Na inactivation relation but simply causes a translation along the voltage axis. 5. It is concluded that a simple surface charge model in which divalent cations do not bind is adequate for Myxicola axons, and that there is no evidence to support a specific involvement of Ca with gating processes.
摘要
- 在电压钳位条件下，对黏液虫巨大轴突在钙离子浓度范围为2至200毫摩尔的溶液中进行了检测。2. 由钙离子浓度变化引起的钠和钾电导-电压曲线的偏移幅度以及膜速率常数是相当的，并且与一个模型相符，该模型中存在 -0.013电荷/埃²的负表面电荷密度，但二价阳离子与外膜表面没有显著结合。3. 用镁等摩尔替代钙对电导-电压曲线沿电压轴的位置没有影响，这与上述结论一致。4. 钙离子对稳态钠失活关系的形状没有影响，只是导致其沿电压轴平移。5. 得出的结论是，对于黏液虫轴突，一个二价阳离子不结合的简单表面电荷模型是足够的，并且没有证据支持钙离子在门控过程中有特定参与。
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本文引用的文献
1
The dual effect of membrane potential on sodium conductance in the giant axon of Loligo.枪乌贼巨大轴突中膜电位对钠电导的双重作用。
J Physiol. 1952 Apr;116(4):497-506. doi: 10.1113/jphysiol.1952.sp004719.
2
A MOLECULAR STRUCTURAL BASIS FOR THE EXCITATION PROPERTIES OF AXONS.轴突兴奋特性的分子结构基础
Biophys J. 1964 May;4(3):167-88. doi: 10.1016/s0006-3495(64)86776-x.
3
The action of calcium on the electrical properties of squid axons.钙对鱿鱼轴突电特性的作用。
J Physiol. 1957 Jul 11;137(2):218-44. doi: 10.1113/jphysiol.1957.sp005808.
4
The effect of changing the internal solution on sodium inactivation and related phenomena in giant axons.改变内部溶液对巨轴突中钠失活及相关现象的影响。
J Physiol. 1965 Oct;180(4):821-36. doi: 10.1113/jphysiol.1965.sp007733.
5
Zeta potential and discrete vs. uniform surface charges.zeta电位以及离散电荷与均匀表面电荷
Biophys J. 1969 Mar;9(3):465-9. doi: 10.1016/S0006-3495(69)86397-6.
6
Effect of divalent cations on potassium conductance of squid axons: determination of surface charge.二价阳离子对鱿鱼轴突钾离子电导的影响：表面电荷的测定
Biophys J. 1969 Mar;9(3):447-63. doi: 10.1016/S0006-3495(69)86396-4.
7
The action of certain polyvalent cations on the voltage-clamped lobster axon.某些多价阳离子对电压钳制的龙虾轴突的作用。
J Gen Physiol. 1968 Mar;51(3):279-91. doi: 10.1085/jgp.51.3.279.
8
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9
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10
Effect of surface charge on the steady-state potassium conductance of nodal membrane.
Nature. 1970 Oct 10;228(5267):164-5. doi: 10.1038/228164a0.
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