Almeida S R, Aroeira L S, Frymuller E, Dias M A, Bogsan C S, Lopes J D, Mariano M
Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, and Center of Electron Microscopy, Federal University of São Paulo, Rua Botucatu 862, 04023-9000, São Paulo, Brazil.
Int Immunol. 2001 Sep;13(9):1193-201. doi: 10.1093/intimm/13.9.1193.
At least three B cell subsets, B-1a, B-1b and B-2, or conventional B cells are present in the mouse periphery. Here we demonstrate that B-1 cells spontaneously proliferate in stationary cultures of normal adherent mouse peritoneal cells. B-1 cells were characterized by morphology, immunohistochemistry and flow cytometry. IgM was detected in the supernatants of these cultures. We demonstrated that the major cell population analyzed expresses the B-1b phenotype. When these cells were transferred to a new culture, a large proportion of them adhere to the plastic surface, and spread as bipolar cells endowed with the capacity to phagocytose via Fc and mannose receptors. Flow cytometry analysis of these adherent cells demonstrated that the great majority of them share both B-220 and Mac-1 antigens. Nevertheless, 45% of them were exclusively Mac-1(+). Finally, when they were labeled in vitro with [(3)H]thymidine and transferred to the peritoneal cavity of naive mice, they migrate to a non-specific inflammatory focus induced by a foreign-body implant. These data demonstrate that B-1 cells, mainly B-1b cells, not only proliferate and differentiate into a mononuclear phagocyte in vitro, but also that they exit the peritoneal cavity and migrate to a non-specific inflammatory milieu.
小鼠外周至少存在三种B细胞亚群，即B-1a、B-1b和B-2或传统B细胞。在此我们证明，B-1细胞在正常贴壁小鼠腹腔细胞的静止培养物中自发增殖。通过形态学、免疫组织化学和流式细胞术对B-1细胞进行了表征。在这些培养物的上清液中检测到了IgM。我们证明，所分析的主要细胞群体表达B-1b表型。当将这些细胞转移到新的培养物中时，其中很大一部分会附着在塑料表面，并作为具有通过Fc和甘露糖受体进行吞噬能力的双极细胞铺展。对这些贴壁细胞的流式细胞术分析表明，它们中的绝大多数同时具有B-220和Mac-1抗原。然而，其中45%仅为Mac-1(+)。最后，当它们在体外用[(3)H]胸腺嘧啶核苷标记并转移到未接触过抗原的小鼠腹腔中时，它们会迁移到由异物植入诱导的非特异性炎症灶。这些数据表明，B-1细胞，主要是B-1b细胞，不仅在体外增殖并分化为单核吞噬细胞，而且它们还离开腹腔并迁移到非特异性炎症环境中。
