Kashiwagi K, Saito K, Wang Y D, Takahashi H, Ishijima K, Tsukahara S
Department of Ophthalmology, Yamanashi Medical University, Yamanashi, Japan.
Ophthalmologica. 2001 Sep-Oct;215(5):351-6. doi: 10.1159/000050884.
To evaluate the safety of an ozonated solution as an antiseptic of the ocular surface prior to ophthalmic surgery.
In experiment 1, a primary culture of rabbit corneal epithelium was established. Then, 0, 4 and 10 ppm ozonated solution and 1.25% povidone-iodine, respectively, were applied to confluent cells on collagen-coated filter inserts (Millicell-CM) for 10 min followed by replacement with fresh medium. The transepithelial electrical resistance (TER), which is a good indicator of cell barrier function, was sequentially measured for 30 min. In experiment 2, adult pigmented rabbit eyes were washed with 20 ml of 4 ppm ozonated solution, 1.25% povidone-iodine solution or saline. Slitlamp examinations were performed before and after washing.
In experiment 1, 4 ppm ozonated solution did not change the TER as compared with the control. 10 ppm ozonated solution and 1.25% povidone-iodine similarly reduced the TER values significantly as compared with those of the control and 4 ppm ozonated solution. In experiment 2, 4 ppm ozonated solution and saline showed mild superficial punctate keratitis (SPK) in 8.3% of eyes. However, 1.25% povidone-iodine resulted in mild SPK in 17% of eyes and moderate SPK in 25% of eyes. The prevalence of SPK between two groups was significantly different (p = 0.03).
Ozonated solution may be safe and a useful antiseptic of the ocular surface prior to ophthalmic surgery.
评估一种臭氧溶液在眼科手术前作为眼表防腐剂的安全性。
在实验1中，建立兔角膜上皮原代培养。然后，分别将0、4和10 ppm的臭氧溶液以及1.25%聚维酮碘应用于胶原包被的滤膜插入物（Millicell-CM）上的汇合细胞10分钟，随后更换新鲜培养基。连续30分钟测量跨上皮电阻（TER），其是细胞屏障功能的良好指标。在实验2中，用20 ml 4 ppm的臭氧溶液、1.25%聚维酮碘溶液或生理盐水冲洗成年有色兔眼。冲洗前后进行裂隙灯检查。
在实验1中，与对照组相比，4 ppm的臭氧溶液未改变TER。与对照组和4 ppm的臭氧溶液相比，10 ppm的臭氧溶液和1.25%聚维酮碘同样显著降低了TER值。在实验2中，4 ppm的臭氧溶液和生理盐水在8.3%的眼中显示轻度浅层点状角膜炎（SPK）。然而，1.25%聚维酮碘在17%的眼中导致轻度SPK，在25%的眼中导致中度SPK。两组之间SPK的患病率有显著差异（p = 0.03）。
臭氧溶液在眼科手术前可能是安全且有用的眼表防腐剂。
