Strawn J R, Ekhator N N, Geracioti T D
Cincinnati Veterans Affairs Medical Center, Department of Psychiatry, OH 45220-2213, USA.
J Chromatogr B Biomed Sci Appl. 2001 Sep 5;760(2):301-6. doi: 10.1016/s0378-4347(01)00290-0.
Cerebrospinal fluid (CSF) concentrations of the monoamine metabolites homovanillic acid (HVA) and 5-hydroxyindolacetic acid (5-HIAA) are commonly used to provide information about central nervous system (CNS) dopaminergic and serotonergic activity. However, little attention has been given to the effects of sample handling on the concentrations of these compounds in human CSF. Using high-performance liquid chromatography (HPLC) with electrochemical detection, we observed that, in CSF stored at -80 degrees C, concentrations of the serotonin metabolite 5-HIAA and the dopamine metabolite HVA remained unchanged through six 1-h and six 24-h freeze-thaw cycles. Exposure to bright room light (3 h, 1,230 lux) resulted in a 5-HIAA concentration that was 96.3 +/- 2.0% of the initial and an HVA concentration that was 98.8 +/- 1.03% of initial. The pH of the CSF significantly increased during both freeze-thaw series and while maintained on ice (4 degrees C). These results demonstrate the in-use stability of 5-HIAA and HVA in human CSF under commonly-encountered laboratory conditions.
脑脊液（CSF）中一元胺代谢产物高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）的浓度通常用于提供有关中枢神经系统（CNS）多巴胺能和5-羟色胺能活性的信息。然而，样本处理对人CSF中这些化合物浓度的影响却很少受到关注。使用高效液相色谱（HPLC）和电化学检测，我们观察到，在-80℃储存的CSF中，5-羟色胺代谢产物5-HIAA和多巴胺代谢产物HVA的浓度在经历六次1小时和六次24小时的冻融循环后保持不变。暴露于明亮的室内光线下（3小时，1230勒克斯），5-HIAA浓度为初始浓度的96.3±2.0%，HVA浓度为初始浓度的98.8±1.03%。在冻融系列过程中以及在冰上保存（4℃）时，CSF的pH值均显著升高。这些结果证明了在常见的实验室条件下，5-HIAA和HVA在人CSF中的使用稳定性。
