聚尿苷酸与固定在硝酸纤维素滤膜上的人类DNA的杂交。
Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.
作者信息
Pero R W, Bryngelsson T, Bryngelsson C, Deutsch A, Nordén A, Norgren A
出版信息
Nucleic Acids Res. 1975 Jul;2(7):1163-76. doi: 10.1093/nar/2.7.1163.
The level of deoxyadenylate (da) regions in human DNA was estimated from formation of poly(U)-poly(da) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 mug/ml at 25 degreesC for 30 min), where as exhaustive ribonuclease treatment (5 mug/ml at 25 degrees C for 6 hr) estimated the more (dA) pure sequences. The level of (dA) rich regions was 0.39% of the DNA and for the more (dA) pure regions it was 0.07%. The (dA) regions were widely distributed throughout human DNA regardless of base composition or sequence repetition. However, a concentration of (dA) regions into main band CsC1 gradient fractions of DNA and into repeated DNA was observed.
通过在耐核糖核酸酶的硝酸纤维素滤膜上形成聚（U）-聚（脱氧腺苷酸）三链体，估算了人类DNA中脱氧腺苷酸（da）区域的水平。富含（dA）的序列是在对聚（U）-DNA杂交体进行温和的核糖核酸酶处理后确定的（25℃下5微克/毫升，处理30分钟），而彻底的核糖核酸酶处理（25℃下5微克/毫升，处理6小时）则用于估算更纯的（dA）序列。富含（dA）区域的水平占DNA的0.39%，对于更纯的（dA）区域，这一比例为0.07%。无论碱基组成或序列重复情况如何，（dA）区域广泛分布于整个人类DNA中。然而，观察到（dA）区域集中在DNA的主带CsC1梯度级分和重复DNA中。
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