Sinnathamby G, Renukaradhya G J, Rajasekhar M, Nayak R, Shaila M S
Department of Microbiology and Cell Biology, Indian Institute of Science, 560 012, Bangalore, India.
Vaccine. 2001 Sep 14;19(32):4816-23. doi: 10.1016/s0264-410x(01)00210-9.
Peste des petits ruminants virus (PPRV), a member of the genus Morbillivirus within the family Paramyxoviridae, causes a fatal disease 'peste des petits ruminants' in goats and sheep. This enveloped virus is antigenically closely related to rinderpest virus (RPV), which causes a similar but distinct disease in large ruminants. PPRV harbors two major surface glycoproteins, the hemagglutinin-neuraminidase (HN) and the fusion (F) proteins. The surface glycoproteins of morbilliviruses are highly immunogenic and confer protective immunity. In this study, we investigated the immune responses generated in goats immunized with low doses of purified recombinant extracellular baculovirus carrying a membrane bound form of the HN protein of PPRV without any adjuvant. We report that the immunized goats develop both humoral and cell-mediated immune responses. Antibodies generated in the immunized animals could neutralize both PPRV and RPV in vitro. Further, using a combination of Escherichia coli expressed deletion mutants of PPRV-HN and RPV-H proteins, and synthetic peptides corresponding to the highly conserved N-terminal sequences of MV-H protein, we have mapped an N-terminal T cell determinant (amino acids 123-137) and a C-terminal domain (amino acids 242-609) harboring potential T cell determinant(s) in goats.
小反刍兽疫病毒（PPRV）是副粘病毒科麻疹病毒属的成员，可在山羊和绵羊中引发致命疾病“小反刍兽疫”。这种有包膜的病毒在抗原性上与牛瘟病毒（RPV）密切相关，牛瘟病毒在大型反刍动物中引发类似但不同的疾病。PPRV含有两种主要的表面糖蛋白，即血凝素神经氨酸酶（HN）和融合（F）蛋白。麻疹病毒的表面糖蛋白具有高度免疫原性并赋予保护性免疫。在本研究中，我们调查了用低剂量纯化的携带PPRV HN蛋白膜结合形式的重组细胞外杆状病毒免疫山羊（无任何佐剂）所产生的免疫反应。我们报告称，免疫山羊产生了体液免疫和细胞介导的免疫反应。免疫动物产生的抗体在体外可中和PPRV和RPV。此外，通过结合使用大肠杆菌表达的PPRV-HN和RPV-H蛋白缺失突变体以及与MV-H蛋白高度保守的N端序列对应的合成肽，我们在山羊中定位了一个N端T细胞决定簇（氨基酸123 - 137）和一个含有潜在T细胞决定簇的C端结构域（氨基酸242 - 609）。
