功能化氙作为一种生物传感器。
Functionalized xenon as a biosensor.
作者信息
Spence M M, Rubin S M, Dimitrov I E, Ruiz E J, Wemmer D E, Pines A, Yao S Q, Tian F, Schultz P G
机构信息
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10654-7. doi: 10.1073/pnas.191368398. Epub 2001 Sep 4.
Abstract
The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capitalizes on the enhanced signal-to-noise, spectral simplicity, and chemical-shift sensitivity of laser-polarized xenon to detect specific biomolecules at the level of tens of nanomoles. We present results using xenon "functionalized" by a biotin-modified supramolecular cage to detect biotin-avidin binding. This biosensor methodology can be extended to a multiplexing assay for multiple analytes.
摘要
生物分子及其相互作用的检测是现代生物医学研究的重要组成部分。在当前的生物传感器技术中，由于信号的光谱重叠，同时检测仅限于少数几种分析物。我们开发了一种基于核磁共振的氙生物传感器，该传感器利用激光极化氙增强的信噪比、光谱简单性和化学位移敏感性，在数十纳摩尔水平上检测特定生物分子。我们展示了使用生物素修饰的超分子笼“功能化”的氙来检测生物素-抗生物素蛋白结合的结果。这种生物传感器方法可以扩展到用于多种分析物的多重检测。
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A Porous Crystalline Molecular Solid Explored by Hyperpolarized Xenon We thank the Italian Ministry of Universities and Scientific Research (PRIN program) for financial support. The research with laser-polarized xenon was supported by the Director, Office of Energy Research, Office of Basic Energy Science, Materials Sciences Division of the U.S. Department of Energy. T.M. thanks the Alexander von Humboldt Foundation for a Feodor Lynen Fellowship.
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