Horn M, Kadgien M, Schnackerz K, Neubauer S
Department of Medicine, University of Würzburg, Germany.
J Cardiovasc Magn Reson. 2000;2(2):143-9. doi: 10.3109/10976640009148684.
31P-nuclear magnetic resonance (NMR) spectroscopy isfrequently used as a tool in the study of organs from various animal species and humans. Because signals arising from the presence of blood are visible in in vivo 31P-NMR spectra of blood-filled organs, such as the heart, it is necessary to correct these spectra for the contribution of blood to the signal. It is unknown whether species differences in 31P signals of blood exist. 31P-containing metabolites of blood from various species were therefore quantified by means of 31P-NMR spectroscopy. Signals of 2,3-bisphosphoglycerate (2,3-DPG); phosphodiesters (PDE); and gamma-, alpha-, and beta-ATP were detected in all 31P-NMR spectra of blood. 2,3-DPG/ATP ratios were significantly higher in dogs, rats, and guinea pigs than in humans but lower in sheep. Pig and rabbit were the only animals with a 2,3-DPG/ATP ratio similar to that of humans. PDE levels varied among species but were significantly lower than in humans only in guinea pigs. The PDE/ATP ratio was relatively similar among all species compared with humans, except dog and guinea pig, where it was significantly higher and lower, respectively. We conclude that because of large species differences, species-specific 31P metabolite ratios should be applied for the correction of in vivo 31P-NMR spectra.
31P-核磁共振（NMR）光谱法经常被用作研究各种动物物种和人类器官的工具。由于血液存在所产生的信号在充满血液的器官（如心脏）的体内31P-NMR光谱中可见，因此有必要对这些光谱进行校正，以消除血液对信号的贡献。目前尚不清楚血液的31P信号是否存在物种差异。因此，通过31P-NMR光谱法对来自不同物种的血液中含31P的代谢物进行了定量分析。在所有血液的31P-NMR光谱中均检测到2,3-二磷酸甘油酸（2,3-DPG）、磷酸二酯（PDE）以及γ-、α-和β-ATP的信号。狗、大鼠和豚鼠的2,3-DPG/ATP比值显著高于人类，但低于绵羊。猪和兔是仅有的2,3-DPG/ATP比值与人类相似的动物。PDE水平在不同物种间有所不同，但仅在豚鼠中显著低于人类。与人类相比，除狗和豚鼠外，所有物种的PDE/ATP比值相对相似，其中狗的该比值显著更高，豚鼠的显著更低。我们得出结论，由于存在较大的物种差异，应应用物种特异性的31P代谢物比值来校正体内31P-NMR光谱。
