Atrih A, Rekhif N, Moir A J, Lebrihi A, Lefebvre G
Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Int J Food Microbiol. 2001 Aug 15;68(1-2):93-104. doi: 10.1016/s0168-1605(01)00482-2.
Plantaricin C19, an anti-Listeria bacteriocin, was successfully purified by adsorption to and release from producing cells at low pH combined with reverse phase high-performance liquid chromatography (HPLC). The purification resulted in a 900-fold increase in specific activity with a yield of 15% of the original activity. Mass spectrometry analysis gave a molecular weight of 3845.3. Protein microsequencing identified 36 amino acids. Plantaricin C19 is rich in both hydrophobic and basic amino acids in good accordance with its basic and hydrophobic character. Comparison of the amino acid sequence of plantaricin C19, with the sequence of some other anti-Listeria bacteriocins produced with lactic acid bacteria, revealed that plantaricin C19 has in its N-terminal region the consensus sequence--YYGNGL--(uniquely with Valine instead of Leucine as found in all other bacteriocins), identifying plantaricin C19 as a pediocin-like bacteriocin. Plantaricin C19 exerted a bacteriostatic action on sensitive cells of Listeria grayi IP 6818 in BHI broth. No loss of intracellular K+, Mg2+ or UV-absorbing materials was observed. Adsorption of plantaricin C19 on L. grayi CIP 6818 decreased in the presence of salts.
植物乳杆菌素C19是一种抗李斯特菌的细菌素，通过在低pH值条件下吸附到产生细胞上并从产生细胞中释放，再结合反相高效液相色谱（HPLC）成功纯化。纯化后比活性提高了900倍，活性回收率为原始活性的15%。质谱分析给出的分子量为3845.3。蛋白质微测序鉴定出36个氨基酸。植物乳杆菌素C19富含疏水氨基酸和碱性氨基酸，与其碱性和疏水特性高度一致。将植物乳杆菌素C19的氨基酸序列与其他一些由乳酸菌产生的抗李斯特菌细菌素的序列进行比较，发现植物乳杆菌素C19在其N端区域具有共有序列——YYGNGL——（唯一不同的是缬氨酸取代了所有其他细菌素中发现的亮氨酸），这表明植物乳杆菌素C19是一种类片球菌素细菌素。植物乳杆菌素C19对BHI肉汤中格氏李斯特菌IP 6818的敏感细胞具有抑菌作用。未观察到细胞内钾离子、镁离子或紫外线吸收物质的损失。在有盐存在的情况下，植物乳杆菌素C19在格氏李斯特菌CIP 6818上的吸附减少。
