Hong S R, Lee S J, Shim J W, Choi Y S, Lee Y M, Song K W, Park M H, Nam Y S, Lee S I
School of Chemical Engineering, Department of Biomedical Engineering, College of Engineering, Hanyang University, Seoul, South Korea.
Biomaterials. 2001 Oct;22(20):2777-83. doi: 10.1016/s0142-9612(01)00021-7.
Gelatin-hyaluronate sponge with and without antibiotic and epidermal growth factor (EGF) were prepared and compared. Four types of sponges were applied on the full-thickness dorsal skin defect of Wistar rat. The effects of antibiotic and EGF in gelatin-hyaluronate sponge on wound healing were investigated by light microscopy and image analyzer at postoperative days of 5, 12 and 21. An immunohistochemical technique, employing PC10, a monoclonal antibody against proliferating cell nuclear antigen (PCNA) was applied to wounded tissue sections. The number of PC10-positive cells was very high for the sponge with EGF at postoperative day 5, then gradually decreased with time. Also we found that antibiotics restrained the cell proliferation during the migratory phase. The sponge with both antibiotic and EGF showed good wound healing performances on the whole for a healing period. The epithelium was regenerated fast with EGF-impregnated sponges at day 5, but each sample had nearly the same length of regenerated epithelium at day 12.
制备并比较了添加和不添加抗生素及表皮生长因子（EGF）的明胶-透明质酸海绵。将四种类型的海绵应用于Wistar大鼠的全层背部皮肤缺损处。在术后第5、12和21天，通过光学显微镜和图像分析仪研究了明胶-透明质酸海绵中抗生素和EGF对伤口愈合的影响。采用抗增殖细胞核抗原（PCNA）的单克隆抗体PC10的免疫组织化学技术应用于受伤组织切片。术后第5天，含EGF的海绵中PC10阳性细胞数量非常高，然后随时间逐渐减少。我们还发现，抗生素在迁移期抑制细胞增殖。含抗生素和EGF的海绵在整个愈合期总体上显示出良好的伤口愈合性能。在第5天，含EGF的海绵使上皮快速再生，但在第12天，每个样本的再生上皮长度几乎相同。
