Levine R, Agren M S, Mertz P M
McGill University, Montreal, Quebec, Canada.
J Cutan Med Surg. 1998 Apr;2(4):193-8. doi: 10.1177/120347549800200403.
Occlusive dressings influence epithelization of superficial wounds by some unknown mechanism(s).
The effects of occlusion on epidermal cell proliferation in two types of wounds were examined.
Partial-thickness wounds and tape-stripped skin wounds were compared. An immunohistochemical technique, employing PC10 - a monoclonal antibody against proliferating cell nuclear antigen (PCNA) - was applied to formalin-fixed, paraffin-embedded porcine tissue sections.
The number of PC10-positive cells was low during the migratory phase, then increased to a peak of proliferation 2 to 3 days after resurfacing. An overall increased proliferative response (mean = 21%) was seen in occluded compared to control partial-thickness wounds (day 10 postoperatively); an opposite effect of occlusion on epidermal proliferation was seen in tape-stripped skin. Occlusion decreased the proliferative response (mean = 42%) compared to air-exposure.
Occlusion increased epidermal cell proliferation in wounds (where the entire surface epithelium and papillary dermis was removed), whereas an opposite effect was seen in tape-stripped skin from which only the stratum corneum had been removed.
封闭性敷料通过某些未知机制影响浅表伤口的上皮形成。
研究封闭对两种伤口表皮细胞增殖的影响。
比较了部分厚度伤口和胶带剥离皮肤伤口。采用免疫组织化学技术，使用PC10（一种针对增殖细胞核抗原（PCNA）的单克隆抗体）对福尔马林固定、石蜡包埋的猪组织切片进行检测。
PC10阳性细胞数量在迁移期较低，然后在创面愈合后2至3天增加至增殖峰值。与对照部分厚度伤口相比（术后第10天），封闭伤口的增殖反应总体增加（平均值 = 21%）；在胶带剥离皮肤中，封闭对表皮增殖有相反的作用。与暴露于空气中相比，封闭降低了增殖反应（平均值 = 42%）。
封闭增加了伤口（整个表面上皮和乳头层真皮被去除的伤口）中的表皮细胞增殖，而在仅去除角质层的胶带剥离皮肤中则观察到相反的效果。
