Hada T, Furuse S, Matsumoto Y, Hamashima H, Masuda K, Shiojima K, Arai T, Sasatsu M
Department of Microbiology, Showa Pharmaceutical University, Machida, Tokyo, Japan.
Microbios. 2001;106 Suppl 2:133-41.
The effects in vitro of tea tree oil (TTO) and plaunotol were examined by monitoring the growth of a standard strain of Staphylococcus aureus FDA 209P and of fourteen methicillin-susceptible strains of S. aureus (MSSA), together with twenty methicillin-resistant strains (MRSA). The minimum inhibitory concentrations (MIC) and the doses for 50% inhibition of growth (ID50) were determined by the micro-broth dilution (MD) method, and the broth dilution with shaking (BDS) method, respectively. The MIC of plaunotol for 50 and 90% of the MSSA and MRSA were assessed by the MD method, as 16 microg/ml and > or = 1,024 microg/ml, respectively. No antibacterial effects of TTO on MSSA and MRSA were detected by the MD method. The growth-inhibitory effects of TTO on S. aureus by the BDS method were examined, and it appeared that TTO was effective over a lower range of concentrations than previously reported. It seems that TTO is very effective in vitro against MSSA and MRSA at high concentrations but less effective below 40 microg/ml of TTO.
通过监测金黄色葡萄球菌标准菌株FDA 209P以及14株甲氧西林敏感金黄色葡萄球菌（MSSA）和20株耐甲氧西林金黄色葡萄球菌（MRSA）的生长情况，研究了茶树油（TTO）和紫苏醇的体外抗菌效果。分别采用微量肉汤稀释（MD）法和振荡肉汤稀释（BDS）法测定最低抑菌浓度（MIC）和50%生长抑制剂量（ID50）。采用MD法评估紫苏醇对50%和90%的MSSA和MRSA的MIC，分别为16微克/毫升和≥1024微克/毫升。MD法未检测到TTO对MSSA和MRSA的抗菌作用。采用BDS法研究了TTO对金黄色葡萄球菌的生长抑制作用，结果表明TTO在比先前报道更低的浓度范围内有效。似乎TTO在体外对MSSA和MRSA在高浓度时非常有效，但在TTO浓度低于40微克/毫升时效果较差。
