Xue Q, Yu Y, Trilk S L, Jong B E, Schumacher M A
Department of Anesthesia and Perioperative Care, The University of California, San Francisco, California, 94143-0648, USA.
Genomics. 2001 Aug;76(1-3):14-20. doi: 10.1006/geno.2001.6582.
Vanilloid receptor subtype-1 (VR1) is a nonselective cation channel that is expressed in sensory neurons and is activated by multiple noxious stimuli. Rat Vr1, stretch-inactivated channel (SIC), and vanilloid receptor 5' splice variant (VR.5'sv) have been hypothesized to be derived from a common VR gene. Characterization of the genomic structure encoding the 5' portion of rat Vr1 confirmed that VR.5'sv is derived from the VR gene; however, SIC seemed to be derived from two related but independent genes. We also deduced the genomic organization of the human gene VR1. Comparative studies of rat and human VR genes showed substantial conservation in genomic organization. The splice site flanking exon-intron 7 in rat and human VR1 diverged from the expected consensus sequence; this may help to explain the skipping of exon 7 within VR.5'sv and other VR splice variants.
香草酸受体亚型1（VR1）是一种非选择性阳离子通道，在感觉神经元中表达，并被多种伤害性刺激激活。大鼠Vr1、伸展失活通道（SIC）和香草酸受体5'剪接变体（VR.5'sv）被推测源自一个共同的VR基因。对编码大鼠Vr1 5'部分的基因组结构的表征证实，VR.5'sv源自VR基因；然而，SIC似乎源自两个相关但独立的基因。我们还推导了人类VR1基因的基因组组织。大鼠和人类VR基因的比较研究表明，基因组组织存在大量保守性。大鼠和人类VR1中外显子-内含子7侧翼的剪接位点与预期的共有序列不同；这可能有助于解释VR.5'sv和其他VR剪接变体中外显子7的跳跃。
