Davis B R, Whitehead J K, Gill M E, Lee P N, Butterworth A D, Roe F J
Br J Cancer. 1975 Apr;31(4):443-52. doi: 10.1038/bjc.1975.84.
In a controlled experiment groups of SPF Wistar rats were give 18 once-fortnightly doses of 0-5, 1-0 or 2-0 mg 3,4-benzpyrene (BP) suspended in infusine (I) or in carbon black (CB) + I by intratracheal instillation. Of rats examined post mortem, 1/16 given I+CB only, 0/16 given I only, 15/51 given BP in I+CB and 24/48 given BP in I developed squamous neoplasms of the lung. The incidence of tumours was significantly related to dose of BP. At the 1 or 2 mg dose levels BP in I only was more productive of tumours than BP in I+CB. Other changes encountered included squamous metaplasia of alveolar and bronchiolar epithelium (Sq.M), but not of bronchial epithelium, and cuboidal and columnar metaplasia of alveolar epithelium in the vicinity of terminal bronchioles (CCM). Sq.M was associated with exposure to BP or I+CB. CCM was strongly associated with exposure to I+CB but only weakly with exposure to BP.
在一项对照实验中，将SPF级Wistar大鼠分为几组，通过气管内滴注给予它们每两周一次、共18次剂量的0.5、1.0或2.0毫克3,4-苯并芘（BP），BP悬浮于输液（I）中或炭黑（CB）+I中。在尸检的大鼠中，仅给予I+CB的大鼠中有1/16发生鳞状肿瘤，仅给予I的大鼠中0/16发生，给予I+CB中BP的大鼠中有15/51发生，给予I中BP的大鼠中有24/48发生。肿瘤发生率与BP剂量显著相关。在1或2毫克剂量水平时，仅I中的BP比I+CB中的BP产生更多肿瘤。遇到的其他变化包括肺泡和细支气管上皮的鳞状化生（Sq.M），但支气管上皮未出现，以及终末细支气管附近肺泡上皮的立方和柱状化生（CCM）。Sq.M与接触BP或I+CB有关。CCM与接触I+CB密切相关，但与接触BP的相关性较弱。
