Effects of asbetos, iron oxide, silica, and carbon black on the microsomal availability of benzo[a]pyrene.

Particulate matter and polynuclear aromatic hydrocarbons are known to be cocarcinogenic. Using fluorescence spectroscopy, we determined that adsorption of benzoic] pyrene (BP) to iron oxide, silica, and asbestos (anthophyllite and Canadian chrysotile) results in a greatly enhanced rate of BP uptake into rat liver microsomes when compared to uptake from aqueous dispersions of BP microcrystals. Simple mixtures of BP microcrystals and particulates do not display enhanced microsomal uptake rates, an observation which indicates that adsorption of BP to the surface of the particle is necessary for enhanced microsomal uptake. BP was not released into microsomes from carbon black. Most importantly, the data indicate that asbestos particles are more effective than silica and iron oxide in enhancing the microsomal availability of BP. These observations suggest that particles, and especially the fibrous mineral particulates, could be cocarcinogenic as a result of their ability to adsorb polynuclear aromatic hydrocarbons and to transport these carcinogens into cells. Except for chrysotile, the particles did not disrupt microsomal integrity as determined by NADPH-dependent lipid peroxidation activity. Binding of the microsomes to the particles did not affect the BP uptake rates. In addition, these BP uptake rates were independent of both the concentrations of microsomes and of particles. These observations are consistent with the mechanism of particle-enhanced transport being an increased rate of BP solubilization from the adsorbed state into the aqueous phase, followed by rapid partitioning of BP into the microsomal membranes.