Imazato Satoshi, Ebi Noboru, Takahashi Yusuke, Kaneko Tomoyuki, Ebisu Shigeyuki, Russell Roy R B
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Biomaterials. 2003 Sep;24(20):3605-9. doi: 10.1016/s0142-9612(03)00217-5.
This study examined the antibacterial activity of prepolymerized resin filler, in which the bactericide quaternary ammonium was immobilized. The experimental filler was prepared by grinding prepolymerized resin blocks of methacrylate monomers, silica particles, and the antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB). The number of Streptococcus mutans after incubation for 18h in contact with the experimental filler with or without protein adsorption by saliva treatment was determined, and adherence of bacteria to the filler surface was evaluated by scanning electron microscopy. Elution of unpolymerized MDPB from the filler and its influence on bacterial growth were also investigated. The growth of S. mutans was completely inhibited by contact with the experimental filler without saliva treatment. Although the effects were attenuated, the saliva-treated filler still exhibited growth inhibition at >99.9%. Less bacteria attached to the experimental filler than the control filler without MDPB, indicating that the reduction in bacterial number after contact with the experimental filler was not due to bacterial adherence to the particles. Unpolymerized MDPB at 1 microg/ml was eluted from the filler particles but was confirmed to have little effect on bacterial growth. The results indicate that the bactericide-immobilized filler containing MDPB shows significant bacteriostatic effects without releasing antibacterial components, and is useful for incorporation into various resin-based restoratives.
本研究检测了固定有杀菌剂季铵盐的预聚合树脂填料的抗菌活性。实验填料是通过研磨甲基丙烯酸酯单体、二氧化硅颗粒和抗菌单体甲基丙烯酰氧基十二烷基溴化吡啶鎓（MDPB）的预聚合树脂块制备而成。测定了经唾液处理有无蛋白质吸附的实验填料与变形链球菌接触18小时后的细菌数量，并通过扫描电子显微镜评估细菌在填料表面的黏附情况。还研究了未聚合的MDPB从填料中的洗脱及其对细菌生长的影响。与未经唾液处理的实验填料接触后，变形链球菌的生长完全受到抑制。尽管效果有所减弱，但经唾液处理的填料仍表现出>99.9%的生长抑制率。与不含MDPB的对照填料相比，附着在实验填料上的细菌更少，这表明与实验填料接触后细菌数量的减少并非由于细菌黏附在颗粒上。1微克/毫升的未聚合MDPB从填料颗粒中洗脱出来，但证实对细菌生长几乎没有影响。结果表明，含有MDPB的固定有杀菌剂的填料在不释放抗菌成分的情况下显示出显著的抑菌作用，可用于各种树脂基修复材料。
