通过hsp65基因的PCR-限制性分析快速区分“卡内蒂分枝杆菌”与其他结核分枝杆菌复合群微生物。
Rapid differentiation of "Mycobacterium canettii" from other Mycobacterium tuberculosis complex organisms by PCR-restriction analysis of the hsp65 gene.
作者信息
Goh K S, Legrand E, Sola C, Rastogi N
机构信息
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, BP 484, F-97165 Pointe-à-Pitre Cedex, Guadeloupe.
出版信息
J Clin Microbiol. 2001 Oct;39(10):3705-8. doi: 10.1128/JCM.39.10.3705-3708.2001.
Abstract
A total of 102 isolates of the Mycobacterium tuberculosis complex, including available "M. canettii" isolates, were studied by PCR-restriction analysis of a 441-bp fragment of the hsp65 gene. PRA upon HhaI enzyme digestion (GCGC) allowed easy differentiation of "M. canettii" from other members of the M. tuberculosis complex (three bands of 260, 105, and 60 bp for "M. canetti," compared to four bands of 185, 105, 75, and 60 bp for other members of the M. tuberculosis complex). Sequencing of the 441-bp hsp65 fragment of "M. canettii" isolates showed the disappearance of an HhaI site at position 235 due to a C-to-T transition that corresponded to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv. Considering that "M. canettii" may also exist as a stable rough morphotype, we suggest that the true number of "M. canettii" isolates may be underestimated in clinical microbiology laboratories.
摘要
对总共102株结核分枝杆菌复合群菌株，包括可得的“堪氏分枝杆菌”菌株，通过对hsp65基因441 bp片段进行PCR-限制性分析来研究。用HhaI酶（GCGC）消化后的PCR-限制性分析能够轻松区分“堪氏分枝杆菌”与结核分枝杆菌复合群的其他成员（“堪氏分枝杆菌”有260、105和60 bp三条带，而结核分枝杆菌复合群的其他成员有185、105、75和60 bp四条带）。对“堪氏分枝杆菌”菌株的441 bp hsp65片段进行测序显示，由于对应于结核分枝杆菌H37Rv同源hsp65基因第631位的C到T转换，第235位的HhaI位点消失。鉴于“堪氏分枝杆菌”也可能以稳定的粗糙形态型存在，我们认为临床微生物学实验室中“堪氏分枝杆菌”菌株的实际数量可能被低估。
相似文献
1
Rapid differentiation of "Mycobacterium canettii" from other Mycobacterium tuberculosis complex organisms by PCR-restriction analysis of the hsp65 gene.通过hsp65基因的PCR-限制性分析快速区分“卡内蒂分枝杆菌”与其他结核分枝杆菌复合群微生物。
J Clin Microbiol. 2001 Oct;39(10):3705-8. doi: 10.1128/JCM.39.10.3705-3708.2001.
2
Study of the gyrB gene polymorphism as a tool to differentiate among Mycobacterium tuberculosis complex subspecies further underlines the older evolutionary age of 'Mycobacterium canettii'.将gyrB基因多态性作为区分结核分枝杆菌复合群亚种的工具进行研究，进一步凸显了“卡内蒂分枝杆菌”更古老的进化年代。
Mol Cell Probes. 2006 Jun-Aug;20(3-4):182-90. doi: 10.1016/j.mcp.2005.11.008. Epub 2006 Mar 6.
3
High genetic diversity revealed by variable-number tandem repeat genotyping and analysis of hsp65 gene polymorphism in a large collection of "Mycobacterium canettii" strains indicates that the M. tuberculosis complex is a recently emerged clone of "M. canettii".通过可变数目串联重复序列基因分型以及对大量“卡内蒂分枝杆菌”菌株的hsp65基因多态性分析所揭示的高遗传多样性表明，结核分枝杆菌复合群是“卡内蒂分枝杆菌”最近出现的一个克隆。
J Clin Microbiol. 2004 Jul;42(7):3248-55. doi: 10.1128/JCM.42.7.3248-3255.2004.
4
5
Description of a novel Mycobacterium simiae allelic variant isolated from Caribbean AIDS patients by PCR-restriction enzyme analysis and sequencing of hsp65 gene.通过PCR-限制性酶切分析和hsp65基因测序对从加勒比地区艾滋病患者中分离出的新型猿分枝杆菌等位基因变体的描述。
Mol Cell Probes. 2000 Dec;14(6):355-63. doi: 10.1006/mcpr.2000.0325.
6
Comparative evaluation of Polymerase Chain Reaction-Restriction Enzyme Analysis (PRA) and sequencing of heat shock protein 65 (hsp65) gene for identification of aquatic mycobacteria.用于鉴定水生分枝杆菌的聚合酶链反应-限制性酶切分析（PRA）与热休克蛋白65（hsp65）基因测序的比较评估
J Microbiol Methods. 2009 Feb;76(2):128-35. doi: 10.1016/j.mimet.2008.09.021. Epub 2008 Oct 10.
7
Simple and rational approach to the identification of Mycobacterium tuberculosis, Mycobacterium avium complex species, and other commonly isolated mycobacteria.鉴定结核分枝杆菌、鸟分枝杆菌复合群菌种及其他常见分离分枝杆菌的简单合理方法。
J Clin Microbiol. 2001 Oct;39(10):3768-71. doi: 10.1128/JCM.39.10.3768-3771.2001.
8
PCR restriction fragment length polymorphism analysis (PRA)-algorithm targeting 644 bp Heat Shock Protein 65 (hsp65) gene for differentiation of Mycobacterium spp.针对644bp热休克蛋白65（hsp65）基因的聚合酶链反应-限制性片段长度多态性分析（PRA）算法，用于分枝杆菌属的鉴别
J Microbiol Methods. 2005 Aug;62(2):199-209. doi: 10.1016/j.mimet.2005.02.010.
9
Comparative evaluation of polymerase chain reaction and restriction enzyme analysis: two amplified targets, hsp65 and rpoB, for identification of cultured mycobacteria.聚合酶链反应与限制性酶切分析的比较评估：用于鉴定培养分枝杆菌的两个扩增靶点，hsp65和rpoB。
Diagn Microbiol Infect Dis. 2005 Mar;51(3):165-71. doi: 10.1016/j.diagmicrobio.2004.09.006.
引用本文的文献
1
Rapid microarray-based assay for detection of pyrazinamide resistant Mycobacterium tuberculosis.基于快速微阵列的结核分枝杆菌吡嗪酰胺耐药检测方法。
Diagn Microbiol Infect Dis. 2019 Jun;94(2):147-154. doi: 10.1016/j.diagmicrobio.2018.12.011. Epub 2018 Dec 30.
2
Smooth Tubercle Bacilli: Neglected Opportunistic Tropical Pathogens.光滑结核分枝杆菌：被忽视的热带机会性病原体。
Front Public Health. 2016 Jan 11;3:283. doi: 10.3389/fpubh.2015.00283. eCollection 2015.
3
Importance of the genetic diversity within the Mycobacterium tuberculosis complex for the development of novel antibiotics and diagnostic tests of drug resistance.结核分枝杆菌复合群内遗传多样性对新型抗生素的开发和耐药性诊断检测的重要性。
Antimicrob Agents Chemother. 2012 Dec;56(12):6080-7. doi: 10.1128/AAC.01641-12. Epub 2012 Sep 24.
4
Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based single nucleotide polymorphism genotyping assay using iPLEX gold technology for identification of Mycobacterium tuberculosis complex species and lineages.基质辅助激光解吸电离飞行时间质谱技术的 iPLEX gold 技术用于单核苷酸多态性基因分型检测，用于鉴定结核分枝杆菌复合群物种和谱系。
J Clin Microbiol. 2011 Sep;49(9):3292-9. doi: 10.1128/JCM.00744-11. Epub 2011 Jul 6.
5
Novel multiplex real-time PCR diagnostic assay for identification and differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis complex strains.新型多重实时 PCR 诊断检测方法，用于鉴定和区分结核分枝杆菌、堪萨斯分枝杆菌和结核分枝杆菌复合菌株。
J Clin Microbiol. 2011 Feb;49(2):651-7. doi: 10.1128/JCM.01426-10. Epub 2010 Dec 1.
6
Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR.应用一种种间可区分的多重 PCR 技术对孟加拉国结核分枝杆菌临床分离株进行鉴定。
BMC Infect Dis. 2010 May 15;10:118. doi: 10.1186/1471-2334-10-118.
7
Distinct genotypic profiles of the two major clades of Mycobacterium africanum.非洲分枝杆菌两个主要分支的独特基因型谱。
BMC Infect Dis. 2010 Mar 29;10:80. doi: 10.1186/1471-2334-10-80.
8
Identification and genotyping of Mycobacterium tuberculosis complex species by use of a SNaPshot Minisequencing-based assay.利用 SNaPshot 微测序法鉴定和基因分型结核分枝杆菌复合群菌种。
J Clin Microbiol. 2010 May;48(5):1758-66. doi: 10.1128/JCM.02255-09. Epub 2010 Mar 10.
9
"Mycobacterium canettii" isolated from a human immunodeficiency virus-positive patient: first case recognized in the United States.从一名人类免疫缺陷病毒阳性患者中分离出的“卡内蒂分枝杆菌”：美国首例确诊病例
J Clin Microbiol. 2009 Jan;47(1):255-7. doi: 10.1128/JCM.01268-08. Epub 2008 Nov 19.
10
A single-step sequencing method for the identification of Mycobacterium tuberculosis complex species.一种用于鉴定结核分枝杆菌复合群物种的单步测序方法。
PLoS Negl Trop Dis. 2008 Jun 18;2(6):e253. doi: 10.1371/journal.pntd.0000253.
本文引用的文献
1
Genetic diversity of Mycobacterium africanum clinical isolates based on IS6110-restriction fragment length polymorphism analysis, spoligotyping, and variable number of tandem DNA repeats.基于IS6110-限制性片段长度多态性分析、间隔寡核苷酸分型及可变数目串联重复序列的非洲分枝杆菌临床分离株的遗传多样性
J Clin Microbiol. 2001 Jan;39(1):57-65. doi: 10.1128/JCM.39.1.57-65.2001.
2
Description of a novel Mycobacterium simiae allelic variant isolated from Caribbean AIDS patients by PCR-restriction enzyme analysis and sequencing of hsp65 gene.通过PCR-限制性酶切分析和hsp65基因测序对从加勒比地区艾滋病患者中分离出的新型猿分枝杆菌等位基因变体的描述。
Mol Cell Probes. 2000 Dec;14(6):355-63. doi: 10.1006/mcpr.2000.0325.
3
A genetic mechanism for deletion of the ser2 gene cluster and formation of rough morphological variants of Mycobacterium avium.鸟分枝杆菌ser2基因簇缺失及粗糙形态变体形成的遗传机制。
J Bacteriol. 2000 Nov;182(21):6177-82. doi: 10.1128/JB.182.21.6177-6182.2000.
4
Genetic variation and evolutionary origin of the direct repeat locus of Mycobacterium tuberculosis complex bacteria.结核分枝杆菌复合群细菌直接重复序列位点的遗传变异与进化起源
J Bacteriol. 2000 May;182(9):2393-401. doi: 10.1128/JB.182.9.2393-2401.2000.
5
hsp65 sequencing for identification of rapidly growing mycobacteria.用于鉴定快速生长分枝杆菌的热休克蛋白65测序
J Clin Microbiol. 1999 Mar;37(3):852-7. doi: 10.1128/JCM.37.3.852-857.1999.
6
Mycobacterium canettii, the smooth variant of M. tuberculosis, isolated from a Swiss patient exposed in Africa.堪内提分枝杆菌，结核分枝杆菌的光滑变种，从一名在非洲接触过的瑞士患者身上分离得到。
Emerg Infect Dis. 1998 Oct-Dec;4(4):631-4. doi: 10.3201/eid0404.980414.
7
Diagnosis of Mycobacterium microti infections among humans by using novel genetic markers.利用新型遗传标记诊断人类微小分枝杆菌感染。
J Clin Microbiol. 1998 Jul;36(7):1840-5. doi: 10.1128/JCM.36.7.1840-1845.1998.
8
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.从全基因组序列解读结核分枝杆菌的生物学特性。
Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
9
Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species.通过hsp65基因的PCR-限制性片段长度多态性分析快速鉴定分枝杆菌至种水平，并提出一种区分34种分枝杆菌的算法。
J Clin Microbiol. 1997 Nov;35(11):2969-73. doi: 10.1128/jcm.35.11.2969-2973.1997.
10
A novel pathogenic taxon of the Mycobacterium tuberculosis complex, Canetti: characterization of an exceptional isolate from Africa.结核分枝杆菌复合群的一种新型致病分类单元——卡内蒂分枝杆菌：一株来自非洲的特殊分离株的特征描述
Int J Syst Bacteriol. 1997 Oct;47(4):1236-45. doi: 10.1099/00207713-47-4-1236.
Zotero 插件