van Soolingen D, van der Zanden A G, de Haas P E, Noordhoek G T, Kiers A, Foudraine N A, Portaels F, Kolk A H, Kremer K, van Embden J D
Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
J Clin Microbiol. 1998 Jul;36(7):1840-5. doi: 10.1128/JCM.36.7.1840-1845.1998.
As a result of DNA typing of Mycobacterium microti isolates from animals in the United Kingdom and The Netherlands, we diagnosed four human M. microti infections. These are the first M. microti infections among humans to be reported. Three of the patients were immunocompromised and suffered from generalized forms of tuberculosis. The fourth patient was a 34-year-old immunocompetent male with a persistent cough and undefined X-ray abnormalities. Two of the M. microti infections were recognized by their IS6110 restriction fragment length polymorphism (RFLP) patterns, which showed a high degree of similarity with those of M. microti strains isolated from a pig and a ferret in The Netherlands. The two other human M. microti infections were recognized by using the recently developed DNA fingerprinting method, "spoligotyping," directly on clinical material. All M. microti isolates from the United Kingdom and The Netherlands were found to contain an exceptionally short genomic direct repeat region, resulting in identical two-spacer sequence reactions in spoligotyping. In contrast, the highly similar IS6110 RFLP patterns of the vole strains from the United Kingdom differed considerably from the RFLPs of all M. microti strains isolated in The Netherlands, suggesting that geographic isolation led to divergent strains in the United Kingdom and on the continent.
通过对英国和荷兰动物体内分离出的微小分枝杆菌菌株进行DNA分型，我们诊断出4例人类微小分枝杆菌感染病例。这些是首次报道的人类微小分枝杆菌感染病例。其中3例患者免疫功能低下，患有全身性结核病。第4例患者是一名34岁免疫功能正常的男性，持续咳嗽，X线检查有不明异常。其中2例微小分枝杆菌感染是通过其IS6110限制性片段长度多态性（RFLP）图谱识别的，这些图谱与从荷兰一头猪和一只雪貂分离出的微小分枝杆菌菌株的图谱高度相似。另外2例人类微小分枝杆菌感染是通过直接对临床材料使用最近开发的DNA指纹图谱方法“间隔寡核苷酸分型法（spoligotyping）”识别的。在英国和荷兰分离出的所有微小分枝杆菌菌株均发现含有一个异常短的基因组直接重复区域，导致间隔寡核苷酸分型法中出现相同的双间隔序列反应。相比之下，英国田鼠菌株高度相似的IS6110 RFLP图谱与在荷兰分离出的所有微小分枝杆菌菌株的RFLP图谱有很大差异，这表明地理隔离导致了英国和欧洲大陆菌株的分化。
