Carroll S F, Martinez R J
Infect Immun. 1979 Sep;25(3):810-9. doi: 10.1128/iai.25.3.810-819.1979.
The antibacterial activity of purified rabbit lysozyme was kinetically investigated at concentrations comparable to those in normal rabbit serum and plasma serum. The bactericidal capability, lysozyme content, and electrophoretic composition of "purified beta-lysin," fractionated from normal rabbit serum, were also examined. In contrast to the extensive antibacterial activity of dilute normal rabbit serum observed in vitro, rabbit lysozyme was only weakly bactericidal for Bacillus subtilis. Inhibition of lysozyme enzymatic and bactericidal activities in normal rabbit serum by antilysozyme immunoglobulin G slightly reduced the initial rate of killing. The addition of neutralizing antibody or histamine (another lysozyme inhibitor) to partially purified bactericidal serum fractions had no effect on killing kinetics. Increasing the ionic strength of reaction mixtures containing normal serum or partially purified bactericidal fractions to levels which completely inhibited lysozyme activity resulted in stimulation of their respective killing kinetics. The addition of inhibitors to normal rabbit plasma serum completely eliminated its bactericidal activity. With regard to the killing of B. subtilis by rabbit and human blood fractions, these analyses clearly demonstrated that (i) although lysozyme is not a significant antibacterial component of normal rabbit serum, it represents the principal factor in normal rabbit plasma serum; (ii) different primary bactericidal mechanisms which are not detectable by singlepoint analyses operate in the sera of different species; and (iii) purified beta-lysin isolated from normal rabbit serum by the classical procedure is a heterogenous mixture of components.
在与正常兔血清和血浆血清中浓度相当的情况下，对纯化的兔溶菌酶的抗菌活性进行了动力学研究。还检测了从正常兔血清中分离得到的“纯化β-溶素”的杀菌能力、溶菌酶含量和电泳组成。与体外观察到的稀释正常兔血清的广泛抗菌活性相反，兔溶菌酶对枯草芽孢杆菌的杀菌作用较弱。抗溶菌酶免疫球蛋白G对正常兔血清中溶菌酶的酶活性和杀菌活性的抑制作用略微降低了初始杀菌速率。向部分纯化的杀菌血清组分中添加中和抗体或组胺（另一种溶菌酶抑制剂）对杀菌动力学没有影响。将含有正常血清或部分纯化杀菌组分的反应混合物的离子强度提高到完全抑制溶菌酶活性的水平，会刺激它们各自的杀菌动力学。向正常兔血浆血清中添加抑制剂会完全消除其杀菌活性。关于兔和人血液组分对枯草芽孢杆菌的杀伤作用，这些分析清楚地表明：（i）虽然溶菌酶不是正常兔血清的重要抗菌成分，但它是正常兔血浆血清中的主要因素；（ii）不同物种血清中存在不同的主要杀菌机制，单点分析无法检测到这些机制；（iii）通过经典方法从正常兔血清中分离得到的纯化β-溶素是一种成分的异质混合物。
