Nishino T, Gallant J, Shalit P, Palmer L, Wehr T
J Bacteriol. 1979 Nov;140(2):671-9. doi: 10.1128/jb.140.2.671-679.1979.
We have examined the accumulation of polyphosphorylated nucleotides in Bacillus subtilis in relation to the function of the rel gene. Our results are as follows. (i) During inhibition of isoleucine activation by O-methylthreonine, wildtype B. subtilis cells accumulate unusual nucleotides with the chromatographic and chemical properties of pppApp, ppApp, pppGpp, ppGpp, pGpp, and ppGp. (ii) During the carbon source downshift elicited by inhibiting glucose uptake, we observed accumulation of the polyphosphorylated guanosine but not adenosine nucleotides. (iii) At the end of long phase in sporulation medium, we observed a small transient accumulation of the polyphosphorylated guanosine but not adenosine nucleotides. (iv) We were unable to detect a nucleotide with chromatographic behavior expected for pppAppp under any conditions. (v) The rel mutant of Swanton and Edlin (Biochem. Biophys. Res. Commun. 46-583-588, 1972) did not accumulate any of these polyphosphorylated nucleotides under any of the conditions examined. (vi) the rel mutant is unimpaired in sporulation. We conclude that one or more of the nucleotides we have detected may be involved in controlling the specificity of transcription during the stringent response, but none of them are required for sporogenesis.
我们研究了枯草芽孢杆菌中多磷酸化核苷酸的积累与rel基因功能的关系。我们的结果如下：（i）在O-甲基苏氨酸抑制异亮氨酸活化过程中，野生型枯草芽孢杆菌细胞积累了具有pppApp、ppApp、pppGpp、ppGpp、pGpp和ppGp色谱和化学性质的异常核苷酸。（ii）在通过抑制葡萄糖摄取引发的碳源降档过程中，我们观察到多磷酸化鸟苷而非腺苷核苷酸的积累。（iii）在芽孢形成培养基的长阶段末期，我们观察到多磷酸化鸟苷而非腺苷核苷酸有少量短暂积累。（iv）在任何条件下，我们都无法检测到具有pppAppp预期色谱行为的核苷酸。（v）Swanton和Edlin的rel突变体（《生物化学与生物物理研究通讯》46:583 - 588，1972）在任何检测条件下都不会积累这些多磷酸化核苷酸中的任何一种。（vi）rel突变体的芽孢形成未受影响。我们得出结论，我们检测到的一种或多种核苷酸可能参与了严谨反应期间转录特异性的控制，但芽孢形成不需要它们中的任何一种。
