[Research on the propagation of Trillium tsehonoskii M. in vitro].

OBJECTIVE

To develop a technique of propagating. Trillium tsehonoskii in vitro as new way to conserve and utilize this special medicinal plant.

METHOD

Segments from different organs of the plant sampled at different growth stages were cultured on MS medium supplemented with various combinations of BA, IAA, NAA and zeatin in a set of concentrations.

RESULT

The effects of the induction of callus and differentiation of sprouts were investigated, showing that BA and IAA favored the formation of callus. The stock segments taken in the period of rapid growth of the plant were suitable for tissue culture. The induction rate of callus from the stock segments was as high as 21.9% and easy to differentiate micro-stock and plantlets, which may result from an activate function of stock cambium to enhance cell division during the stage of sprouting to rapid growth.

CONCLUSION

The tissue culture method is a practicable and potential biotechnological way to multiply Trillium tsehonoskii plants. One key point to establish an in vitro system is to further improve the plantlet differentiation rate of the callus induced.