• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Detection of cytomegalovirus DNA in human specimens by LightCycler PCR: melting point analysis is mandatory to detect virus strains with point mutations in the target sequence of the hybridization probes.

作者信息

Schaade L, Kockelkorn P, Ritter K, Kleines M

出版信息

J Clin Microbiol. 2001 Oct;39(10):3809. doi: 10.1128/JCM.39.10.3809.2001.

DOI:10.1128/JCM.39.10.3809.2001
PMID:11599519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC88443/
Abstract
摘要

相似文献

1
Detection of cytomegalovirus DNA in human specimens by LightCycler PCR: melting point analysis is mandatory to detect virus strains with point mutations in the target sequence of the hybridization probes.采用LightCycler PCR技术检测人标本中的巨细胞病毒DNA:对于检测杂交探针靶序列中存在点突变的病毒株,熔解温度分析必不可少。
J Clin Microbiol. 2001 Oct;39(10):3809. doi: 10.1128/JCM.39.10.3809.2001.
2
LightCycler-based quantitative PCR for detection of cytomegalovirus in blood, urine, and respiratory samples.基于LightCycler的定量PCR技术用于检测血液、尿液和呼吸道样本中的巨细胞病毒。
J Clin Microbiol. 2001 Jun;39(6):2364-5. doi: 10.1128/JCM.39.6.2364-2365.2001.
3
PCR detection of human cytomegalovirus DNA in clinical specimens using novel UL37 exon 3 and US3 primers.使用新型UL37外显子3和US3引物对临床标本中的人巨细胞病毒DNA进行PCR检测。
Clin Diagn Lab Immunol. 1998 Mar;5(2):256-8. doi: 10.1128/CDLI.5.2.256-258.1998.
4
In situ PCR to cells and to wax sections. An alternative to in situ hybridization for the detection of low copies of human papillomavirus and cytomegalovirus DNA.
Methods Mol Biol. 1997;71:77-97. doi: 10.1385/0-89603-395-3:77.
5
Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients.用于检测骨髓移植受者巨细胞病毒感染的定量血浆PCR平板检测法的评估
J Clin Microbiol. 1997 Mar;35(3):788-90. doi: 10.1128/jcm.35.3.788-790.1997.
6
Detection of cytomegalovirus DNA in human specimens by LightCycler PCR.运用LightCycler PCR技术检测人类样本中的巨细胞病毒DNA。
J Clin Microbiol. 2000 Nov;38(11):4006-9. doi: 10.1128/JCM.38.11.4006-4009.2000.
7
Application of PCR in situ method for detection of human cytomegalovirus (HCMV) DNA.聚合酶链反应原位法在检测人巨细胞病毒(HCMV)DNA中的应用。
Acta Microbiol Pol. 2001;50(2):169-73.
8
Optimization of human cytomegalovirus LightCycler real-time PCR.人巨细胞病毒LightCycler实时聚合酶链反应的优化
J Infect Dev Ctries. 2008 Oct 1;2(5):406-10. doi: 10.3855/jidc.208.
9
Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction.通过聚合酶链反应对人巨细胞病毒序列进行酶促扩增。
J Clin Microbiol. 1989 Aug;27(8):1802-9. doi: 10.1128/jcm.27.8.1802-1809.1989.
10
Detection of human cytomegalovirus polymerase chain reaction products using oligonucleotide probes directly conjugated to alkaline phosphatase.使用直接与碱性磷酸酶偶联的寡核苷酸探针检测人巨细胞病毒聚合酶链反应产物。
J Virol Methods. 1996 Sep;61(1-2):145-50. doi: 10.1016/0166-0934(96)02081-2.

引用本文的文献

1
WU Polyomavirus (WUPyV): A Recently Detected Virus Causing Respiratory Disease?WU 多瘤病毒(WUPyV):一种新发现的引起呼吸道疾病的病毒?
Viruses. 2009 Dec;1(3):678-88. doi: 10.3390/v1030678. Epub 2009 Nov 4.
2
Real-time PCR diagnostics failure caused by nucleotide variability within exon 4 of the human cytomegalovirus major immediate-early gene.人巨细胞病毒主要立即早期基因外显子4内的核苷酸变异性导致实时PCR诊断失败。
J Clin Microbiol. 2007 Mar;45(3):1042-4. doi: 10.1128/JCM.01109-06. Epub 2007 Jan 17.
3
Errors in published sequences of human cytomegalovirus primers and probes: do we need more quality control?已发表的人类巨细胞病毒引物和探针序列中的错误:我们是否需要加强质量控制?
J Clin Microbiol. 2005 Oct;43(10):5408-9. doi: 10.1128/JCM.43.10.5408-5409.2005.
4
Sequence diversity in the glycoprotein B gene complicates real-time PCR assays for detection and quantification of cytomegalovirus.糖蛋白B基因中的序列多样性使巨细胞病毒检测和定量的实时PCR检测变得复杂。
J Clin Microbiol. 2005 Oct;43(10):4968-71. doi: 10.1128/JCM.43.10.4968-4971.2005.
5
Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens.三种用于检测拭子标本中单纯疱疹病毒的LightCycler聚合酶链反应检测方法的初步比较
Eur J Clin Microbiol Infect Dis. 2003 Dec;22(12):764-7. doi: 10.1007/s10096-003-1031-2. Epub 2003 Nov 7.
6
Comparison of LightCycler-based PCR, COBAS amplicor CMV monitor, and pp65 antigenemia assays for quantitative measurement of cytomegalovirus viral load in peripheral blood specimens from patients after solid organ transplantation.基于LightCycler的聚合酶链反应、COBAS扩增仪巨细胞病毒监测仪和pp65抗原血症检测法在实体器官移植患者外周血标本中巨细胞病毒病毒载量定量测定的比较。
J Clin Microbiol. 2003 Jul;41(7):3167-74. doi: 10.1128/JCM.41.7.3167-3174.2003.

本文引用的文献

1
Detection of cytomegalovirus DNA in human specimens by LightCycler PCR.运用LightCycler PCR技术检测人类样本中的巨细胞病毒DNA。
J Clin Microbiol. 2000 Nov;38(11):4006-9. doi: 10.1128/JCM.38.11.4006-4009.2000.