Tiwari A K, Srinivas P V, Kumar S P, Rao J M
Division of Pharmacology and Natural Product Laboratory and Division of Organic Chemistry-I, Indian Institute of Chemical Technology, Hyderabad 500 007, India.
J Agric Food Chem. 2001 Oct;49(10):4642-5. doi: 10.1021/jf010573a.
An activity-directed fractionation and purification process was used to identify the antioxidant components of Cedrus deodara. Dried heartwood powder of C. deodara was first defatted with petroleum ether and then extracted with chloroform. The chloroform extract showed strong antioxidant activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. This fraction was then subjected to separation and purification using silica gel column chromatography. Three compounds with potent antioxidant activity were isolated in significant yields and identified by spectroscopic methods ((1)H NMR, (13)C NMR, IR, and MS). They were identified as (-)-matairesinol, (-)-nortrachelogenin, and a dibenzylbutyrolactollignan (4,4',9-trihydroxy-3,3'-dimethoxy-9,9'-epoxylignan). This is the first report of the occurrence of these compounds in C. deodara.
采用活性导向的分离纯化方法来鉴定喜马拉雅雪松的抗氧化成分。首先用石油醚对喜马拉雅雪松的心材干粉进行脱脂，然后用氯仿萃取。氯仿提取物对1,1-二苯基-2-苦基肼（DPPH）自由基表现出较强的抗氧化活性。接着，该馏分通过硅胶柱色谱进行分离纯化。以较高产率分离出三种具有强抗氧化活性的化合物，并通过光谱方法（1H NMR、13C NMR、IR和MS）进行鉴定。它们被鉴定为（-）-罗汉松脂素、（-）-去甲落叶松脂素和一种二苄基丁内酯木脂素（4,4',9-三羟基-3,3'-二甲氧基-9,9'-环氧木脂素）。这是首次报道这些化合物在喜马拉雅雪松中的存在。
