来自薏苡仁（Coix lachryma-jobi L. var. ma-yuen Stapf）外壳的2,2'-二苯基-1-苦基肼自由基清除活性成分。
2,2'-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls.
作者信息
Kuo Ching-Chuan, Chiang Wenchang, Liu Guey-Ping, Chien Ya-Lin, Chang Jang-Yang, Lee Ching-Kuo, Lo Jir-Mehng, Huang Shou-Ling, Shih Ming-Chih, Kuo Yueh-Hsiung
机构信息
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan, Republic of China.
出版信息
J Agric Food Chem. 2002 Oct 9;50(21):5850-5. doi: 10.1021/jf020391w.
An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods ((1)H NMR, (13)C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).
采用活性导向的分级分离和纯化工艺来鉴定薏苡仁壳的抗氧化成分。用甲醇提取薏苡仁（Coix lachryma-jobi L. var. ma-yuen Stapf）的外壳，然后分离成水相、正丁醇相、乙酸乙酯相和己烷相。与水相、乙酸乙酯相和己烷相可溶部分相比，正丁醇可溶部分表现出更强的清除2,2'-二苯基-1-苦基肼（DPPH）自由基的能力。然后，正丁醇部分通过Diaion HP-20柱色谱、硅胶柱色谱和高效液相色谱进行分离和纯化。通过光谱方法（1H NMR、13C NMR、红外光谱和质谱）并与标准品比较，鉴定出6种具有强抗氧化活性的化合物，分别为松柏醇（1）、丁香酸（2）、阿魏酸（3）、丁香树脂醇（4）、4-酮松脂醇（5）和一种新的木脂素——美原内酯（6）。
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