Luo D, Xu W, Liang G, Wang S, Wang Z, Bi Z, Zhu W
Institute of Dermatology, CAMS & PUMC, 12 Jiang Wangmiao Road, Nanjing 210042, China.
Chin Med J (Engl). 1999 Jun;112(6):489-92.
To investigate Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) in China by means of culture, Mg-DNA detection, observation under an electron microscope (EM) and an atomic force microscope (AFM).
227 urogenital specimens taken from both patients in clinics and high risk populations of STDs in Beijing, Nanjing and Shanghai areas were cultured in SP-4 medium for isolation of Mg. Suspicious positive strains were identified first by PCR technique which could amplify the special DNA fragments of Mg adhesion protein (Mg-Pa) and 16S-rRNA sequences. The morphologic features of Mg were observed under EM and AFM.
Eight strains were isolated from the 227 specimens, the total positive rate was 3.52%. The isolated strains met the criteria of growth and metabolic conditions required by Mg. Specific fragments of Mg-DNA (374 bp based on Mg-Pa and 402 bp on 16S-rRNA primers, respectively) could be amplified from both the isolates and the genital secretions. Under EM and AFM, Mg showed different shapes, including flask, pear, spindle and globe shape. The typical shape of Mg was flask or pear shape with projecting neck portion and broadened terminal structure under EM and with constrictive ring between the neck and body under AFM.
Mg infection exists in high risk populations of STDs in China. We highly suggest the use of AFM in dermatological study.
通过培养、Mg-DNA检测、电子显微镜（EM）及原子力显微镜（AFM）观察，研究中国性传播疾病（STD）高危人群中的生殖支原体（Mg）感染情况。
取自北京、南京和上海地区诊所患者及STD高危人群的227份泌尿生殖标本，在SP-4培养基中培养以分离Mg。首先通过PCR技术鉴定可疑阳性菌株，该技术可扩增Mg黏附蛋白（Mg-Pa）和16S-rRNA序列的特殊DNA片段。在EM和AFM下观察Mg的形态特征。
从227份标本中分离出8株菌株，总阳性率为3.52%。分离出的菌株符合Mg所需的生长和代谢条件标准。从分离株和生殖分泌物中均可扩增出Mg-DNA的特异性片段（基于Mg-Pa引物的为374 bp，基于16S-rRNA引物的为402 bp）。在EM和AFM下，Mg呈现不同形状，包括烧瓶形、梨形、纺锤形和球形。Mg的典型形状在EM下为颈部突出、末端结构变宽的烧瓶形或梨形，在AFM下颈部与主体之间有收缩环。
中国STD高危人群中存在Mg感染。我们强烈建议在皮肤病学研究中使用AFM。
