Garthwaite I, Ross K M, Miles C O, Briggs L R, Towers N R, Borrell T, Busby P
AgResearch Ruakura, Toxinology and Food Safety Group, Hamilton, New Zealand.
J AOAC Int. 2001 Sep-Oct;84(5):1643-8.
Enzyme-linked immunosorbent assays (ELISAs) were developed for amnesic, neurotoxic, and diarrhetic shellfish poisoning (ASP, NSP, and DSP) toxins and for yessotoxin. These assays, along with a commercially available paralytic shellfish poisoning (PSP) ELISA, were used to test the feasibility of an ELISA-based screening system. It was concluded that such a system to identify suspect shellfish samples, for subsequent analysis by methods approved by international regulatory authorities, is feasible. The assays had sufficient sensitivity and can be used on simple shellfish extracts. Alcohol extraction gave good recovery of all toxin groups. The ease of ELISAs permits the ready expansion of the system to screen for other toxins, as new ELISAs become available.
已开发出用于检测失忆性、神经毒性和腹泻性贝类毒素（ASP、NSP和DSP）以及虾夷毒素的酶联免疫吸附测定（ELISA）法。这些测定法与一种市售的麻痹性贝类毒素（PSP）ELISA一起，用于测试基于ELISA的筛选系统的可行性。得出的结论是，这样一个用于识别可疑贝类样本以便随后通过国际监管机构批准的方法进行分析的系统是可行的。这些测定法具有足够的灵敏度，可用于简单的贝类提取物。酒精提取能使所有毒素组都获得良好的回收率。ELISA操作简便，随着新的ELISA法问世，该系统可方便地扩展用于筛查其他毒素。
