The basic requirements for the function of the isolated cell free perfused rat kidney.

We have attempt to define experimental conditions which would overcome or minimize some of the well known functional limitations of isolated single pass kidney preparations. Rat kidneys were perfused with a Krebs-Henseleit solution containing the gelatine derivative Haemaccel as colloid. Perfusion was initiated in situ via the mesenteric artery. Arterial flow rate was measured continuously from the very onset of perfusion. Effective perfusion pressure was recorded distal to the perfusion capillary in the aorta. Aliquots of the venous effluate and of an arterial bypass solution were drawn through an O-2 electrode for the calculation of Q-o-2. First it was shown that the often observed initial vasoconstriction of the preparation which occurs immediately after cannulation of the kidney can be eliminated by rapid disconnection of the autonomic nerve supply. A more delayed gradual increase of renal resistance, which we observed after 30 min could be prevented by using sterile perfusion solutions. Using glucose as the only substrate fuel, fractional Na-reabsorption decreased to 65% 3 hrs after the onset of perfusion (T Na equals 27.3 muEq/g with min). When a substrate enriched sterile solution was used containing pyruvate, lactate, oxaloacetate, and glutamate, Na conservation of the isolated kidney could be maintained at a higher level. Fractional Na-reabsorption levelled off and was still 88% after 3 hrs (T Na equals 64.4 muEq/g with min). The results demonstrate that the transport function of the isolated kidney preparation critically depends on the supply with substrate hydrogen. Thus, the present system meets the basic requirements necessary for further micropuncture evaluation of renal function under the condition of isolated single pass perfusion.