Manian S, Sreenivasaprasad S, Bending G D, Mills P R
Department of Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UK.
FEMS Microbiol Lett. 2001 Oct 16;204(1):117-21. doi: 10.1111/j.1574-6968.2001.tb10873.x.
The nucleotide sequence of the internal transcribed spacer (ITS) 1 and 2 regions was determined for 22 Suillus isolates representing seven species. Analysis of the sequence data along with 12 related sequences from databases divided the 34 isolates into seven groups, and six of these were supported by 100% bootstrap values. Isolates belonging to Suillus granulatus (0-11 divergence) were divided into three groups (SG1, SG2 and SG3); SG1 and SG2 had 100% bootstrap values. SG2 was clustered with two Suillus collinitus isolates and a Suillus variegatus isolate; SG3 was linked to a previously characterised Suillus subluteus isolate. These results clearly indicate heterogeneity among the isolates described under these species. Intra- (0.4-11) and inter- (2.4-13.6) specific divergence varied considerably. Suillus laricinus showed a high level (0.5-4.4) of intra-specific divergence. Suillus bovinus showed maximum divergence (11.4-13.3) to all other species examined. The potential of the ITS region for understanding the intra- and inter-specific relationships among Suillus species and refining their taxonomy is discussed.
测定了代表7个物种的22个滑锈伞属分离株的内转录间隔区（ITS）1和2区域的核苷酸序列。将序列数据与来自数据库的12个相关序列进行分析，把34个分离株分为7组，其中6组得到了100%的自展值支持。属于颗粒滑锈伞（差异为0 - 11）的分离株被分为3组（SG1、SG2和SG3）；SG1和SG2的自展值为100%。SG2与两个粘柄滑锈伞分离株和一个杂色滑锈伞分离株聚类在一起；SG3与一个先前鉴定的淡黄滑锈伞分离株相关联。这些结果清楚地表明了这些物种名下分离株之间的异质性。种内（0.4 - 11）和种间（2.4 - 13.6）差异变化很大。落叶松滑锈伞显示出较高水平（0.5 - 4.4）的种内差异。牛肝滑锈伞与所有其他被检测物种的差异最大（11.4 - 13.3）。讨论了ITS区域在理解滑锈伞属物种内和种间关系以及完善其分类学方面的潜力。
