Piesman J, Schneider B S, Zeidner N S
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Public Health Service, Fort Collins, Colorado 80522, USA.
J Clin Microbiol. 2001 Nov;39(11):4145-8. doi: 10.1128/JCM.39.11.4145-4148.2001.
Quantitative real-time PCR was used to assay spirochetes in feeding ticks. Spirochetes in tick midguts increased sixfold, from 998 per tick before attachment to 5,884 at 48 h of attachment. Spirochetes in tick salivary glands increased >17-fold, from 1.2 per salivary gland pair before feeding to 20.8 at 72 h postattachment. The period of the most rapid increase in the number of spirochetes in the salivary glands occurred from 48 to 60 h postattachment; this time period coincides with the maximal increase in transmission risk during nymphal tick feeding.
采用定量实时聚合酶链反应(Quantitative real-time PCR)检测正在吸血的蜱体内的螺旋体。蜱中肠内的螺旋体数量增加了6倍,从附着前每只蜱998个增加到附着48小时后的每只蜱5884个。蜱唾液腺内的螺旋体数量增加超过17倍,从吸血前每对唾液腺1.2个增加到附着后72小时的每对唾液腺20.8个。唾液腺内螺旋体数量增加最快的时期发生在附着后48至60小时;这个时间段与若蜱吸血期间传播风险的最大增加相吻合。